Sf-FGFR and Sf-SR-C Are Not the Receptors for Vip3Aa to Exert Insecticidal Toxicity in Spodoptera frugiperda.
Sf-FGFR and Sf-SR-C Are Not the Receptors for Vip3Aa to Exert Insecticidal Toxicity in Spodoptera frugiperda.
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CRISPR/Cas9基因编辑和生化分析表明，敲除Sf-FGFR或Sf-SR-C基因不会改变草地贪夜蛾对Vip3Aa的敏感性。 Vip3Aa 是苏云金芽孢杆菌 (Bt) 在营养生长阶段分泌的一种新型杀虫蛋白。对草地贪夜蛾等鳞翅目害虫具有较高的杀虫活性，与Cry杀虫蛋白无交叉抗性。作为一种新型杀虫剂，在防治农业害虫方面发挥着重要作用。然而，Vip3Aa毒素的杀虫机制，特别是其明确的受体，尚未完全揭示。本研究利用CRISPR/Cas9系统分别敲除先前报道的Vip3Aa受体基因Sf-FGFR和Sf-SR-C。生物测定结果表明，这两个基因敲除菌株对Vip3Aa的敏感性与正常菌株相比没有明显变化。目前的结果与之前报道的Sf-SR-C和Sf-FGFR是Vip3Aa的体外受体不一致。这表明我们测试的 Sf-SR-C 和 Sf-FGFR 基因对于草地贪夜蛾体内 Vip3Aa 的作用模式可能并不重要。
The CRISPR/Cas9 gene editing and biochemical analysis show that knocking out the Sf-FGFR or Sf-SR-C gene will not change the sensitivity of Spodoptera frugiperda to Vip3Aa. Vip3Aa is a novel insecticidal protein secreted by Bacillus thuringiensis (Bt) during its vegetative growth stages. It has high insecticidal activity against lepidopteran pests such as Spodoptera frugiperda, and has no cross-resistance with Cry insecticidal proteins. As a new type of insecticide, it plays an important role in controlling agricultural pests. However, the insecticidal mechanism of the Vip3Aa toxin, especially its definite receptors, have not been fully revealed. In this study, the previously reported Vip3Aa receptor genes Sf-FGFR and Sf-SR-C were knocked out separately using the CRISPR/Cas9 system. Bioassay results showed that the sensitivity of these two knockout strains to Vip3Aa were not significantly changed compared to that of the normal strain. The current results are not consistent with the previously reports that Sf-SR-C and Sf-FGFR were the receptors of Vip3Aa in vitro. This suggests that the Sf-SR-C and Sf-FGFR genes we tested may not be critical in the mode of action of Vip3Aa in vivo in Spodoptera frugiperda.