Gene repression using synthetic small regulatory RNA in Methylorubrum extorquens
Gene repression using synthetic small regulatory RNA in Methylorubrum extorquens
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DOI：
10.1111/jam.15159
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发表时间：
2021-05
影响因子：
4
通讯作者：
Li-Ping Zhu;Sunmi Song;Song Yang
中科院分区：
文献类型：
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作者：
Li-Ping Zhu;Sunmi Song;Song Yang
遗传工具是为合成生物学和生物技术设计细胞工厂的先决条件。扭脱甲基红菌是未来一碳（C1）生物经济的重要平台，但其应用目前受到遗传工具可用性的限制。小分子调控RNA（Small regulatory RNA，sRNA）是细菌中一种重要的调控因子，在许多细菌中被用来抑制基因表达。本研究旨在构建一个基于MicC支架和分子伴侣Hfq的合成sRNA系统来控制M.扭体
Genetic tools are a prerequisite for engineering cell factories for synthetic biology and biotechnology. Methylorubrum extorquens is an important platform for a future one‐carbon (C1) bioeconomy, but its application is currently limited by the availability of genetic tools. Small regulatory RNA (sRNA) is an important regulatory factor in bacteria and has been applied for gene repression in several strains. This study aimed to construct a synthetic sRNA system based on the MicC scaffold and the chaperone Hfq to control gene expression in M. extorquens.