Employing multi-omics to elucidate the hormetic response against oxidative stress exerted by nC(60) on Daphnia pulex
Employing multi-omics to elucidate the hormetic response against oxidative stress exerted by nC(60) on Daphnia pulex
复制标题
采用多组学来阐明 nC(60) 对水蚤产生的氧化应激的激效反应
DOI：
10.1016/j.envpol.2019.04.097
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发表时间：
2019
影响因子：
8.9
通讯作者：
Ong Choon Nam
中科院分区：
文献类型：
--
作者：
Wang Pu;Ng Qinxiang;Zhang Bo;Wei Zhikai;Hassan Muhammad;He Yiliang;Ong Choon Nam
采用转录组学和代谢组学方法研究富勒烯晶体（nC 60）对蚤状蚤（Daphnia pulex）的氧化应激效应。氧化应激的激素效应在7天后最明显，低剂量nC 60暴露时L-谷胱甘肽（GSH）含量和超氧化物歧化酶（SOD）活性显著增加，而高剂量则相反。本研究采用转录组学和代谢组学的方法，探讨了氧化应激中毒物兴奋效应的分子机制。蚤状蚤的氧化应激和能量代谢的主要途径发生了显著变化。一些重要的中间产物及其调控基因的表达量随着浓度的增加呈现先上调后下调的趋势，符合毒物兴奋效应的描述。nC 60干扰蚤状蚤TCA循环。直接影响L-半胱氨酸和谷氨酸的合成，并进一步干扰GSH的合成。本研究为深入研究nC 60对紫球藻氧化应激的兴奋效应提供了分子水平的证据。
This study evaluated hormetic effect of oxidative stress exerted by fullerene crystals (nC60) onDaphnia pulex, employingtranscriptomics and metabolomics.D. pulexwere exposed to various concentrations of nC60for 21 days. Hormetic effect of oxidative stress was most evident after 7 days, with markedly increased L-Glutathione (GSH) concentration and Superoxide Dismutase (SOD) activity at low doses of nC60exposure, and oppositely at high doses. The transcriptomics and metabolomics were used to elucidate the molecular mechanism underlying the hormesis in oxidative stress. There were significant alterations in major pathways involving oxidative stress and energy metabolism inD. pulex. Some important intermediates and the expression of their regulatory genes coincided with each other with first up-regulated and then down-regulated with the concentration increased, consistent with the hormesis description. The nC60interfered the TCA cycle ofD. pulex. The synthesis of L-cysteine and glutamate was directly affected, and further disturbed the synthesis of GSH. This work is of great significance to provide the molecular-level evidence into the hormetic effect in oxidative stress ofD. pulexexposed to nC60.