Quantitation of haemopoietic cells from normal and leukaemic RFM mice using an in vivo colony assay.
Quantitation of haemopoietic cells from normal and leukaemic RFM mice using an in vivo colony assay.
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使用体内菌落测定法对正常和白血病RFM小鼠的造血细胞进行定量。
DOI：
10.1038/bjc.1974.216
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发表时间：
1974-11
影响因子：
8.8
通讯作者：
Gordon, M Y
中科院分区：
文献类型：
--
作者：
Gordon, M Y
Benestad（1970）描述的传统扩散室（CDC）已被改进，以测定RFM骨髓和脾脏细胞在琼脂扩散室（adc）中的集落形成能力。菌落在形态上与体外琼脂培养中CFUc形成的菌落相同，其发生率约为103个正常有核骨髓细胞中有1个，104个有核脾细胞中有1个。正常骨髓细胞在CDCs和adc中的生长情况的比较表明，细胞在扩散室中的增殖可能是由琼脂培养中发现的集落形成的同一前体细胞引起的。用3h标记的胸腺嘧啶孵育后，大约46%的ADC集落前体细胞自杀，这一建议得到了支持。来自白血病小鼠的造血细胞的集落形成似乎是由于剩余正常细胞群的增殖，而接种物中的白血病细胞形成均匀分布的母细胞背景。在白血病细胞培养的情况下，cdc和ADC的结果存在差异，白血病ADC培养菌落的数据与正常ADC菌落的数据相似。这些比较暗示ADC技术可用于监测正常骨髓的功能能力，通过其形成菌落的能力，在白血病的发展。白血病环境对adc中正常骨髓细胞生长的体液效应也被检测到。
The conventional diffusion chamber (CDC) as described by Benestad (1970) had been modified to assay the colony forming capacity of RFM bone marrow and spleen cells in agar diffusion chambers (ADCs). The colonies are morphologically identical to those formed by the CFUc in agar culture in vitro and have an incidence of approximately 1 in 103 normal nucleated bone marrow cells, and 1 in 104 nucleated spleen cells. Comparison of the growth of normal bone marrow cells in CDCs and in ADCs suggests that cell proliferation in diffusion chambers may result from the same precursor cell as detected by colony formation in agar culture in vitro. This proposal is supported by the suicide of approximately 46% of the ADC colony precursor cells following incubation with 3H-labelled thymidine. Colony formation by haemopoietic cells taken from leukaemic mice appears to be due to the proliferation of a remaining normal cell population alone, while the leukaemic cells in the inoculum form a background of uniformly distributed blast cells. In the case of leukaemic cell culture, there are differences in the results from CDCs and ADCs, and data from colonies in leukaemic ADC cultures are similar to those from normal ADC colonies. These comparisons imply that the ADC technique may be used to monitor the functional capacity of normal bone marrow, by its ability to form colonies, during the development of leukaemia. A humoral effect of a leukaemic environment on the growth of normal bone marrow cells in ADCs has also been detected.