Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection
Simultaneous determination of nine flavonoids and qualitative evaluation of Herba Epimedii by high performance liquid chromatography with ultraviolet detection
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DOI：
10.1002/jssc.200700262
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发表时间：
2007-12-01
影响因子：
3.1
通讯作者：
Zhang, Weidong
中科院分区：
文献类型：
--
作者：
Huang, Hao;Liang, Mingjin;Zhang, Weidong
建立了淫羊藿中9种黄酮类化合物（槲皮素-3-O-葡萄糖苷、朝藿定A、己苷A、朝藿定B、朝藿定C、淫羊藿苷、淫羊藿苷1、淫羊藿苷11和淫羊藿苷）的高效液相色谱测定方法。在Diamonsil C-18柱（5 μ m，250 x 4.6 mm）上进行色谱分离，用乙腈-0.5%醋酸缓冲液（用三乙胺调节至pH 5.00）梯度洗脱。方法学验证得到了可接受的线性（r（2）>0.9992）和回收率（98.9 ~ 102.4%）。这些黄酮类化合物的检测限为16.3 - 83.3 ng。结果表明，不同产地淫羊藿中黄酮类化合物含量差异较大，含量范围为0.05 ~ 39.0mg/g。采用所建立的方法对5种不同产地的25个淫羊藿样品进行了分析，结果表明，不同产地和不同来源的药材对淫羊藿的质量有一定的影响。该方法简便、有效，适用于该中药的质量评价。
A simple high performance liquid chromatographic method has been developed for the determination of nine flavonoids in Herba Epimedii, including quercetin-3-O-glucoside, epimedin A, hexandraside A, epimedin B, epimedin C, icariin, icariside 1, icariside 11, and icaritin. The chromatographic separation was performed on a Diamonsil C-18 column (5 mu m, 250 x 4.6 mm) with gradient elution by acetonitrile-0.5% acetic buffer (adjusted to pH 5.00 with triethylamine). Methodological validation gave acceptable linearities (r(2) >0.9992) and recoveries (ranging from 98.9 to 102.4%). The limits of detection of these flavonoids ranged from 16.3 to 83.3 ng. The results indicated that the contents of flavonoids in Herba Epimedii varied significantly from habitat to habitat with contents ranging from 0.05 to 39.0 mg/g. Twenty five Herba Epimedii samples prepared from five different botanical materials were investigated by the established method, and the results showed the influence of the botanical material and the habitat on the quality of Herba Epimedii. The proposed method is simple, effective, and suitable for the evaluation of this traditional Chinese medicine.