Kruppel-like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning
Kruppel-like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning
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DOI：
10.1002/dvdy.24310
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发表时间：
2015-10-01
影响因子：
2.5
通讯作者：
Cao, Ying
中科院分区：
文献类型：
--
作者：
Gao, Yan;Cao, Qing;Cao, Ying
Kruppel样因子（Klfs）是哺乳动物中由17个成员组成的转录因子家族，Klf 1-Klf 17，其参与基本的细胞生理过程，如细胞增殖、分化和凋亡。然而，它们在胚胎发育中的功能却知之甚少。我们前期的研究表明，多能性因子Klf 4通过调控关键的发育信号，参与非洲爪蟾胚胎的胚层形成和轴的形成。在本研究中，我们进一步全面研究了klf家族基因klf 2，klf 5，klf 6，klf 7，klf 8，klf 11，klf 15和klf 17在非洲爪蟾胚胎发生过程中的表达和功能。时空表达分析表明，这些基因在非洲爪蟾胚胎的母系和合子转录，并在器官发生和组织分化过程中，它们被定位到各种基板和组织。功能获得和丧失的研究表明，Klf因子在胚层形成和体轴模式中发挥不同的作用。此外，每个Klf因子对胚层形成和体轴图案形成所必需的基因的表达表现出不同的调节作用。这些结果表明，Klf因子参与了这些基因在早期胚胎发生过程中的微调。发展动力学244：1328-1346，2015年。(c)2015 Wiley Periodicals，Inc.
Kruppel-like factors (Klfs) are a family of transcription factors consisting of 17 members in mammals, Klf1-Klf17, which are involved in fundamental cellular physiological procedures, such as cell proliferation, differentiation, and apoptosis. However, their functions in embryonic development have been poorly understood. Our previous study has demonstrated that the pluripotency factor Klf4 participates in germ layer formation and axis patterning of Xenopus embryos by means of the regulation of key developmental signals. In the present study, we further investigated comprehensively the expression and functions of the klf family genes, klf2, klf5, klf6, klf7, klf8, klf11, klf15, and klf17, during the embryogenesis of Xenopus laevis. Spatio-temporal expression analyses demonstrate that these genes are transcribed both maternally and zygotically in Xenopus embryos, and during organogenesis and tissue differentiation, they are localized to a variety of placodes and tissues. Gain and loss of function studies manifest that Klf factors play different roles in germ layer formation and body axis patterning. Moreover, each Klf factor exhibits distinct regulatory effects on the expression of genes that are essential for germ layer formation and body axis patterning. These results suggest that Klf factors are involved in the fine-tuning of these genes during early embryogenesis. Developmental Dynamics 244:1328-1346, 2015. (c) 2015 Wiley Periodicals, Inc.