Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography
Analysis of phenolic compounds by high performance liquid chromatography and ultra performance liquid chromatography
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DOI：
10.1016/j.talanta.2008.02.021
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发表时间：
2008-06-30
期刊：
影响因子：
6.1
通讯作者：
Solich, Petr
中科院分区：
文献类型：
--
作者：
Spacil, Zdenek;Novakova, Lucie;Solich, Petr
本文建立了两种基于亚2微米颗粒色谱柱的酚类化合物分析新方法。HPLC系统配备C-18硅胶分析柱（50 mm x 4.6 mm，1.8 μ m）和UPLC系统配备乙烯桥连杂化C-18分析柱（100 mm x 2.1 mm，1.7 μ m）。通过10次重复进样，测定各梯度方法的系统适用性试验数据，包括保留时间和峰面积的重复性、不对称因子的平均值、分离度、峰容量和理论塔板的高度当量。所开发的方法应用于真实的样品（葡萄酒，茶）的分析。(C)2008 Elsevier B. V.保留所有权利。
Two novel chromatographic methods both based on utilization of sub-2-micron particle columns were developed for the analysis of phenolic compounds in this work. An HPLC system was equipped with C-18 silica-based analytical column (50 mm x 4.6 mm, 1.8 mu m) and a UPLC system with ethylene-bridged hybrid C-18 analytical column (100 mm x 2.1 mm, 1.7 mu m).In total 34 phenolic substances were divided into groups of phenolic acids, flavonoids, catechins and coumarins and were analysed in sequence using different gradient methods. System suitability test data, including repeatability of retention time and peak area, mean values of asymmetry factor, resolution, peak capacity and the height equivalent of a theoretical plate were determined for each gradient method by 10 replicate injections. The developed methods were applied in the analysis of real samples (grape wines, teas). (C) 2008 Elsevier B.V. All rights reserved.