Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications
Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications
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口腔鳞状细胞癌中profilin 2表达的降低及其临床病理意义
DOI：
10.3892/or.2011.1365
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发表时间：
2011-10-01
期刊：
影响因子：
4.2
通讯作者：
Han, W.
中科院分区：
文献类型：
--
作者：
Ma, C. Y.;Zhang, C. P.;Han, W.
Profilin 是许多正常细胞动力学所必需的小蛋白质，并且是基于肌动蛋白的细胞运动的关键组成部分之一。最近的几项研究表明 profilin (PFN) 家族在癌症发病机制和进展中的作用。然而，它们在口腔鳞状细胞癌（OSCC）中的表达和有希望的功能在很大程度上是未知的。在本研究中，我们分析了 PFN1 和 PFN2 体外和体内表达的相关性。使用质谱法粗略比较细胞系（HIOEC、HB96）之间的蛋白质表达水平。 PFN2 被选为癌性 HB96 细胞中显着下调的基因之一。通过RT-PCR、实时PCR和Western blotting验证PFN1和PFN2的体外表达水平。首次使用激光扫描共聚焦显微镜评估 PFN2 表达的定位。在随后的实验中，我们观察了PFN2表达水平与转染的HB96癌细胞增殖的关系。还在 PFN2 转染或未转染的 HB96 细胞中检查了 VASP、N-WASP 和 P27 表达。在体内，通过免疫组织化学分析确定 88 个配对组织样本中的抗原表达。与癌旁正常粘液上皮相比，88 名 OSCC 患者的癌组织中证实了蛋白表达降低。 PFN2 表达较弱的肿瘤与强表达肿瘤相比，预后显着较差（P
Profilins are small proteins essential for many normal cellular dynamics and constitute one of the crucial components of actin-based cellular motility. Several recent studies have implicated a role for the profilin (PFN) family in cancer pathogenesis and progression. However, their expression and promising functions are largely unknown in oral squamous cell carcinoma (OSCC). In this study, we analyzed the correlation between PFN1 and PFN2 expression in vitro and in vivo. The protein expression levels were roughly compared between cell lines (HIOEC, HB96) with the employment of mass spectrometry. PFN2 was singled out as one of the significantly down-regulated genes in the cancerous HB96 cells. The expression levels of PFN1 and PFN2 in vitro were validated by RT-PCR, real-time PCR and Western blotting. Laser scanning confocal microscopy was used for the first time to assess the localization of PFN2 expression. In subsequent experiments, we observed the relationship between PFN2 expression levels and the proliferation of transfected HB96 cancer cells. VASP, N-WASP and P27 expression was also examined in the PFN2-transfected or non-transfected HB96 cells. In vivo, antigen expression was determined by immunohistochemical analyses in 88 paired tissue specimens. Decreased protein expression was confirmed in cancerous tissues from 88 OSCC patients compared with paracancerous normal mucous epithelia. Tumors with weak PFN2 expression were associated with a significantly worse prognosis than strongly expressed tumours (P