A PROTEIN-KINASE ANTIGENICALLY RELATED TO PP60V-SRC POSSIBLY INVOLVED IN YEAST-CELL CYCLE CONTROL - POSITIVE INVIVO REGULATION BY STEROL
A PROTEIN-KINASE ANTIGENICALLY RELATED TO PP60V-SRC POSSIBLY INVOLVED IN YEAST-CELL CYCLE CONTROL - POSITIVE INVIVO REGULATION BY STEROL
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DOI：
10.1073/pnas.84.12.4012
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发表时间：
1987-06-01
影响因子：
11.1
通讯作者：
DAHL, J
中科院分区：
文献类型：
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作者：
DAHL, C;BIEMANN, HP;DAHL, J
在酿酒酵母的甾醇营养缺陷型中检查了麦角甾醇（酵母的天然甾醇）对细胞周期和与 pp60v-src 抗原相关的蛋白激酶的影响。甾醇耗尽的细胞以未出芽的 G1 状态积累。添加非限制性麦角甾醇或胆固醇与微量麦角甾醇后，细胞出芽和增殖重新启动，而单独添加胆固醇或微量麦角甾醇则效果较差。与酵母蛋白和抗 pp60v-src 免疫复合物相关的蛋白激酶的刺激显示与添加麦角甾醇后退出 G1 期呈正相关。麦角甾醇刺激的细胞还表现出磷脂酰肌醇激酶活性的增加。数据表明，麦角甾醇的激素水平（有效浓度，约1nM）参与与可能参与酵母细胞周期控制的蛋白激酶相关的信号传导过程。
The effects of ergosterol, yeast''s natural sterol, on cell cycling and a protein kinase antigenically related to pp60v-src were examined in a sterol auxotroph of Saccharomyces cerevisiae. Sterol-depleted cells accumulate in an unbudded, G1 state. Cell budding and proliferation are reinitiated upon addition of nonlimiting ergosterol or cholesterol with trace ergosterol, whereas cholesterol or trace ergosterol alone is less effective. Stimulation of a protein kinase associated with immune complexes of yeast protein and anti-pp60v-src shows a positive correlation with exit from the G1 phase following ergosterol addition. Ergosterol-stimulated cells also demonstrate an increase in phosphatidylinositol kinase activity. The data suggest that hormonal levels of ergosterol (effective concentration, .apprxeq. 1 nM) participate in a signaling process associated with a protein kinase possibly involved in yeast cell cycle control.