Multicenter observational study on slow-cooling oocyte cryopreservation: clinical outcome
Multicenter observational study on slow-cooling oocyte cryopreservation: clinical outcome
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DOI：
10.1016/j.fertnstert.2009.10.029
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发表时间：
2010-10-01
影响因子：
6.7
通讯作者：
Scaravelli, Giulia
中科院分区：
文献类型：
--
作者：
Borini, Andrea;Setti, Paolo Emanuele Levi;Scaravelli, Giulia
目的：目的：评价在冷冻液中加入0.2mol/L蔗糖的单次缓慢降温法冷冻卵母细胞的效果。设计图：新鲜和冷冻解冻周期的临床结果的观察比较。设置：公共和私人IVF中心。患者：接受IVF治疗的不孕夫妇。干预：根据当地法律规定，新鲜周期中最多使用三个卵母细胞，冷冻保存并随后使用多余的卵母细胞。同样，新鲜周期，每个周期使用最多三个解冻的卵母细胞。所有解冻的卵母细胞进行microinjected.Main Outcome Measure（s）：新鲜和解冻cycles. Results（s）的胚胎学和临床参数：2046例患者接受了2,209个涉及卵母细胞冷冻保存的卵母细胞回收。总体而言，解冻卵母细胞的存活率为55.8%。在940个解冻周期中，与新鲜周期结果相比，平均授精卵母细胞数和受精率显著降低（分别为2.6 +/- 0.7 vs. 2.9 +/- 0.2和72.5% vs. 78.3%），植入率也是如此（10.1% vs. 15.4%）、每次移植的妊娠率（17.0% vs. 27.9%）和每个周期的妊娠率（13.7% vs. 26.2%）。不同中心的临床结局存在差异。大多数诊所每个解冻周期的妊娠率均超过14%。57例涉及卵母细胞冷冻保存的检索在新鲜胚胎置换后实现了妊娠。每次胚胎移植和每次解冻周期的植入率和妊娠率分别为17.5%、28.6%和24.6%，respectively. Conclusions（s）：在测试条件下，与新鲜周期相比，卵母细胞缓冷冷冻保存的临床结局降低。然而，在胚胎冷冻不适用的情况下，应向有多余卵母细胞的患者提供卵母细胞冷冻。（Fertil Steril（R）2010;94：1662-8. (C)美国生殖医学会（American Society for Reproductive Medicine）
Objective: To evaluate the efficacy of oocyte cryopreservation by a single slow-cooling protocol involving sucrose (0.2 mol/L) in the freezing solution. Design: Observational comparison of the clinical outcome in fresh and frozen thawed cycles.Setting: Public and private IVF centers.Patient(s): Infertile couples undergoing IVF treatment.Intervention(s): Use of a maximum three oocytes in fresh cycles, as established by local law, and cryopreservation and later use of surplus oocytes. Likewise fresh cycles, maximum three thawed oocytes were used per cycle. All thawed oocytes were microinjected.Main Outcome Measure(s): Embryologic and clinical parameters of fresh and thawed cycles.Result(s): Two thousand forty-six patients underwent 2,209 oocyte retrievals involving oocyte cryopreservation. Overall, the survival rate of thawed oocytes was 55.8%. In 940 thaw cycles, the mean numbers of inseminated oocytes and fertilization rates were significantly decreased vs. fresh cycles outcomes (2.6 +/- 0.7 vs. 2.9 +/- 0.2 and 72.5% vs. 78.3%, respectively), as were the rates of implantation (10.1% vs. 15.4%), pregnancy rates per transfer (17.0% vs. 27.9%), and pregnancy rates per cycle (13.7% vs. 26.2%). Differences in clinical outcome were found among centers. A pregnancy rate per thawing cycle above 14% was achieved by most clinics. Fifty-seven retrievals involving oocyte cryopreservation achieved a pregnancy after fresh embryo replacement. Implantation and pregnancy rates per embryo transfer and per thawing cycles were 17.5%, 28.6%, and 24.6%, respectively.Conclusion(s): Under the conditions tested, the clinical outcome of oocyte slow-cooling cryopreservation is reduced compared with fresh cycles. Nevertheless, in cases of inapplicability of embryo cryopreservation, oocyte cryopreservation should be offered to patients with surplus oocytes. (Fertil Steril (R) 2010;94:1662-8. (C) 2010 by American Society for Reproductive Medicine.)