The embryonic central nervous system lineages of Drosophila melanogaster .2. Neuroblast lineages derived from the dorsal part of the neuroectoderm
The embryonic central nervous system lineages of Drosophila melanogaster .2. Neuroblast lineages derived from the dorsal part of the neuroectoderm
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DOI：
10.1006/dbio.1997.8660
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发表时间：
1997-09-15
影响因子：
2.7
通讯作者：
Technau, GM
中科院分区：
文献类型：
--
作者：
Schmidt, H;Rickert, C;Technau, GM
在果蝇中，中枢神经系统（CNS）的形成始于神经外胚层的分层，每个半节约有30个神经母细胞（NBs）。它们在胚胎发育过程中产生大约350个神经元和30个神经胶质细胞。理解导致中枢神经系统细胞命运规范和分化的机制需要识别NE谱系。来自神经外胚层腹侧部分的17个NBs的胚胎谱系已经被描述过（Bossing et al., 1996）。在这里，我们提出了来自神经外胚层背侧部分的13个谱系，并将其中12个分配给已识别的NBs。总的来说，这13个谱系包括大约120个神经元和22到27个神经胶质细胞，我们将其包括在一个系统术语中。因此，来自背神经外胚层的NBs产生了胚胎腹神经节中约90%的胶质细胞。两种NBs只产生神经胶质子代（NB 6-4A， G；P），五种NBs 1-3, 2-5, 5-6, 6-4T, 7-4)。这7个nb在网元层最外侧的区域排成一组。其他谱系（NBs 2-4、3-3、3-5、4-3、4-4、5-4、克隆y）完全由神经元（中间神经元、运动神经元，或两者兼而有之）组成。此外，甚至跳过表达细胞（EL）的侧簇可能与ne3 -3谱系相关联。随着先前描述的克隆，绝大多数（超过90%）胚胎腹侧神经索（胸、腹）的细胞系现在是已知的。此外，以前鉴定的神经元和大多数神经胶质细胞现在与某些谱系有关，因此与特定的NBs有关。这一完整的数据集为突变表型的解释和未来对细胞命运规范和分化的研究提供了基础。(C) 1997学术出版社。
In Drosophila, central nervous system (CNS) formation starts with the delamination from the neuroectoderm of about 30 neuroblasts (NBs) per hemisegment. They give rise to approximately 350 neurons and 30 glial cells during embryonic development. Understanding the mechanisms leading to cell fate specification and differentiation in the CNS requires the identification of the NE lineages. The embryonic lineages derived from 17 NBs of the ventral part of the neuroectoderm have previously been described (Bossing et al., 1996). Here we present 13 lineages derived from the dorsal part of the neuroectoderm and we assign 12 of them to identified NBs. Together, the 13 lineages comprise approximately 120 neurons and 22 to 27 glial cells which we include in a systematic terminology. Therefore, NBs from the dorsal neuroectoderm produce about 90% of the glial cells in the embryonic ventral ganglion. Two of the NBs give rise to glial progeny exclusively (NB 6-4A, G;P) and five to glia as well as neurons (NBs 1-3, 2-5, 5-6, 6-4T, 7-4). These seven NBs are arranged as a group in the most lateral region of the NE layer. The other lineages (NBs 2-4, 3-3, 3-5, 4-3, 4-4, 5-4, clone y) are composed exclusively of neurons (interneurons, motoneurons, or both). Additionally, it has been possible to link the lateral cluster of even-skipped expressing cells (EL) to the lineage of NE 3-3. Along with the previously described clones, the vast majority (more than 90%) of cell lineages in the embryonic ventral nerve cord (thorax, abdomen) are now known. Moreover, previously identified neurons and most glial cells are now Linked to certain lineages and, thus, to particular NBs. This complete set of data provides a foundation for the interpretation of mutant phenotypes and for future investigations on cell fate specification and differentiation. (C) 1997 Academic Press.