Steroidogenesis in the gonads of rainbow trout fry (Salmo gairdneri) before and after the onset of gonadal sex differentiation.
Steroidogenesis in the gonads of rainbow trout fry (Salmo gairdneri) before and after the onset of gonadal sex differentiation.
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虹鳟鱼苗（Salmo gairdneri）性腺性别分化开始之前和之后性腺的类固醇生成。
DOI：
10.1051/rnd:19820313
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Jan Peute
中科院分区：
文献类型：
--
作者：
R. Hurk;J.G.D. Lambert;Jan Peute
受精后D（ay）60、D 100和D 200虹鳟鱼性腺匀浆在脱氢表雄酮-3H存在下体外孵育，以证明3 β-羟基类固醇脱氢酶（3 β-HSD）和δ 5，4-异构酶活性，的双烯醇酮-3H评估“孕激素”和雄烯二酮的合成，3 H测定卵巢中雌激素的合成和睾丸中雄激素的形成。从D50开始的组织学上无关的性腺含有3 β-HSD、δ 5，4-异构酶和17 α-羟化酶，表明有合成孕激素的能力。来自D100的卵巢具有与D50性腺相同的酶;来自D200的卵巢还具有17 β-HSD和芳香化酶，指示雌激素合成。与D50性腺不同，D100睾丸还含有17 α，20-碳链酶和11 β-羟化酶，显示出合成雄激素的能力。17 β-HSD仅在D200睾丸中表现出。本文讨论了雄激素和孕激素在虹鳟性腺性别分化调控中的可能作用。超微结构和酶化学研究表明，间质（Leydig）细胞作为来源的类固醇在虹鳟鱼睾丸从D100起。在这些细胞中，具有许多管状嵴的大线粒体的出现与11 β-羟化酶活性的出现同步。在年轻的性腺和发育中的卵巢中不能证明明显的类固醇生成位点。
Gonadal homogenates of rainbow trout from D(ay) 60, D100 and D200 after fertilization have been incubated in vitro in the presence of dehydroepiandrosterone-3H to demonstrate 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and delta 5,4-isomerase activity, of pregnenolone-3H to assess the synthesis of "progestins" and of androstenedione-3H to determine oestrogen synthesis in the ovaries and the formation of androgens in the testes. Histologically indifferent gonads from D50 contained 3 beta-HSD, delta 5,4-isomerase and 17 alpha-hydroxylase, indicating a capacity to synthesize progestins. Ovaries from D100 possessed the same enzymes as D50 gonads; those from D200 had, in addition, 17 beta-HSD and aromatase, indicative of oestrogen synthesis. Unlike the D50 gonads, the D100 testes also contained 17 alpha, 20-desmolase and 11 beta-hydroxylase, showing a capacity to synthesize androgens. 17 beta-HSD was only demonstrated in D200 testes. The possible role of androgens and progestins in the regulation of gonadal sex differentiation in rainbow trout has been discussed. Ultrastructural and enzymecytochemical investigations have demonstrated stroma (Leydig) cells as sources of steroidogenesis in rainbow trout testis from D100 onwards. The appearance of big mitochondria with many tubular cristae in those cells synchronized with the appearance of 11 beta-hydroxylase activity. Obvious steroidogenic sites could not be demonstrated in younger gonads and developing ovaries.