H9N2 influenza virus isolated from minks has enhanced virulence in mice
H9N2 influenza virus isolated from minks has enhanced virulence in mice
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从水貂身上分离出的H9N2流感病毒对小鼠的毒力增强
DOI：
10.1111/tbed.12805
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发表时间：
2018-06-01
影响因子：
4.3
通讯作者：
Liu, Q.
中科院分区：
文献类型：
--
作者：
Xue, R.;Tian, Y.;Liu, Q.
H9 N2是我国禽流感病毒的主要亚型之一，其宿主范围广泛，从鸟类到哺乳动物。2014年从一个水貂养殖场分离到两株H9 N2病毒。系统进化分析表明，H9 N2病毒内部基因与H7N9病毒内部基因有较近的亲缘关系。有趣的是，两个H9 N2在系统发育树中被分离，表明它们是在两个独立的事件中被引入这个水貂养殖场的。进一步的小鼠研究表明，一种H9 N2病毒在感染小鼠中引起明显的体重减轻和20%的死亡率，而另一种病毒在相同剂量下感染小鼠时没有引起任何临床症状。遗传分析表明，强毒株H9 N2在PB 2蛋白的701 N处存在一个天然突变，该突变与哺乳动物的适应性有关。然而，在对小鼠无毒力的H9 N2中不存在这种取代。H9 N2在水貂中的传播可能会驱使病毒适应哺乳动物;有必要对水貂中的流感病毒进行持续监测。
H9N2 is one of the major subtypes of influenza virus circulating in poultry in China, which has a wide host range from bird to mammals. Two H9N2 viruses were isolated from one mink farm in 2014. Phylogenetic analysis showed that internal genes of the H9N2 viruses have close relationship with those of H7N9 viruses. Interestingly, two H9N2 were separated in phylogenetic trees, indicating that they are introduced to this mink farm in two independent events. And further mice studies showed that one H9N2 caused obvious weight loss and 20% mortality in infected mice, while another virus did not cause any clinical sign in mice infected at the same dose. Genetic analysis indicated that the virulent H9N2 contain a natural mutation at 701N in PB2 protein, which was reported to contribute to mammalian adaptation. However, such substitution is absent in the H9N2 avirulent to mice. Circulation of H9N2 in mink may drive the virus to adapt mammals; continual surveillance of influenza virus in mink was warranted.