Single-cell analysis of sodium channel expression in dorsal root ganglion neurons.
Single-cell analysis of sodium channel expression in dorsal root ganglion neurons.
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DOI：
10.1016/j.mcn.2010.08.017
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发表时间：
2011-01
影响因子：
3.5
通讯作者：
O'Leary, Michael E.
中科院分区：
文献类型：
--
作者：
Ho, Cojen;O'Leary, Michael E.
背根神经节（DRG）的感觉神经元表达多个电压门控钠（Na）通道，这些通道在门控动力学和药理学方面有很大差异。从大鼠DRG分离的小直径（<25 µm）神经元表达快速河豚毒素敏感（TTX-S）和慢TTX-R）Na电流的组合，而大直径神经元（>30 µm）主要表达快速TTX-S Na电流。使用单细胞RT-PCR进一步研究Na通道表达，以测量单独收获的DRG神经元中存在的转录物。与细胞电生理学一致，小神经元表达编码TTX-S（Nav1.1，Nav1.2，Nav1.6，Nav1.7）和TTX-R（Nav1.8，Nav1.9）Na通道的转录物。Nav1.7、Nav1.8和Nav1.9是在小神经元中表达的主要Na通道。大神经元高表达TTX-S亚型（Nav1.1、Nav1.6、Nav1.7），而TTX-R通道以相对低的水平存在。一个独特的亚群的大神经元被确定为表达TTX-R钠电流和高水平的Nav1.8转录。DRG神经元在神经丝（NF 200，外周蛋白）和Necl-1（一种参与髓鞘形成的神经元粘附分子）的表达方面也显示出实质性差异。NF 200和Necl-1的优先表达表明大直径神经元产生厚的有髓轴突。小直径的神经元表达外周蛋白，但NF 200和Necl-1的水平降低，这种模式与薄的无髓鞘轴突更一致。Na通道转录本的单细胞分析表明，TTX-S和TTX-R Na通道在大的有髓鞘（Nav1.1，Nav1.6，Nav1.7）和小的无髓鞘（Nav1.7，Nav1.8，Nav1.9）感觉神经元中差异表达。
Sensory neurons of the dorsal root ganglia (DRG) express multiple voltage-gated sodium (Na) channels that substantially differ in gating kinetics and pharmacology. Small-diameter (<25 µm) neurons isolated from the rat DRG express a combination of fast tetrodotoxin-sensitive (TTX-S) and slow TTX-resistant (TTX-R) Na currents while large-diameter neurons (>30 µm) predominately express fast TTX-S Na current. Na channel expression was further investigated using single-cell RT-PCR to measure the transcripts present in individually harvested DRG neurons. Consistent with cellular electrophysiology, the small neurons expressed transcripts encoding for both TTX-S (Nav1.1, Nav1.2, Nav1.6, Nav1.7) and TTX-R (Nav1.8, Nav1.9) Na channels. Nav1.7, Nav1.8 and Nav1.9 were the predominant Na channels expressed in the small neurons. The large neurons highly expressed TTX-S isoforms (Nav1.1, Nav1.6, Nav1.7) while TTX-R channels were present at comparatively low levels. A unique subpopulation of the large neurons was identified that expressed TTX-R Na current and high levels of Nav1.8 transcript. DRG neurons also displayed substantial differences in the expression of neurofilaments (NF200, peripherin) and Necl-1, a neuronal adhesion molecule involved in myelination. The preferential expression of NF200 and Necl-1 suggests that large-diameter neurons give rise to thick myelinated axons. Small-diameter neurons expressed peripherin, but reduced levels of NF200 and Necl-1, a pattern more consistent with thin unmyelinated axons. Single-cell analysis of Na channel transcripts indicates that TTX-S and TTX-R Na channels are differentially expressed in large myelinated (Nav1.1, Nav1.6, Nav1.7) and small unmyelinated (Nav1.7, Nav1.8, Nav1.9) sensory neurons.
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影响因子：
5.5
作者：
ELLIOTT, AA;ELLIOTT, JR
通讯作者：
ELLIOTT, JR
影响因子：
2.5
作者：
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通讯作者：
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作者：
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作者：
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Yasuda, H
DOI：
10.1073/pnas.95.15.8963
发表时间：
1998-07-21
影响因子：
11.1
作者：
Dib-Hajj, SD;Tyrrell, L;Waxman, SG
通讯作者：
Waxman, SG