USE OF CYPRIDINA LUCIFERIN ANALOG FOR ASSESSING THE MONOAMINE OXIDASE-LIKE SUPEROXIDE-GENERATING ACTIVITIES OF TWO PEPTIDE SEQUENCES CORRESPONDING TO THE HELICAL COPPER-BINDING MOTIF IN HUMAN PRION PROTEIN AND ITS MODEL ANALOG
USE OF CYPRIDINA LUCIFERIN ANALOG FOR ASSESSING THE MONOAMINE OXIDASE-LIKE SUPEROXIDE-GENERATING ACTIVITIES OF TWO PEPTIDE SEQUENCES CORRESPONDING TO THE HELICAL COPPER-BINDING MOTIF IN HUMAN PRION PROTEIN AND ITS MODEL ANALOG
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使用荧光素荧光素类似物评估人朊病毒蛋白中与螺旋铜结合基序相对应的两个肽序列的单胺氧化酶样超氧化物生成活性及其模型类似物
DOI：
10.1142/9789812839589_0019
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
T. Kawano
中科院分区：
文献类型：
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作者：
Ken Yokawa;T. Kagenishi;T. Kawano
在这项研究中，我们比较了对应于PrP螺旋Cu结合位点的两种肽序列的O2-产生活性，即生物化学研究中经常使用的模型类似物序列，34和天然PrP中发现的原始序列，用海萤属植物蛋白类似物（CLA）作为荧光探针，研究了PrP螺旋Cu结合区的模型序列使用Brown等人<$提出的（VNITKQHTVTTTT），因为该序列显示出在某些芳族单胺存在下作为产生O2的催化剂是活性的。3此外，还制备了对应于天然螺旋序列VNITIKQHTVTTTT的肽（图1）。化学合成两种肽，并由Sigma Genosis Japan（Ishikari，北海道）在HPLC上纯化（纯度分别为99.20%、99.10%）。
In this study, we compared the O2"-generating activity of two peptide sequences corresponding to the PrP helical Cu-binding site namely the model analog sequence often employed in the biochemical studies, 34 and the original sequence found in the native PrP, using Cypridina luciferin analog (CLA) as a chemiluminescent probe for O₂MATERIALS The model sequence for PrP helical Cu-binding region (VNITKQHTVTTTT) proposed by Brown et al. ¹ was used since this sequence was shown to be active as the catalyst for generation of O2 in presence of some aromatic monoamines. 3 Additionally, a peptide corresponding to the natural helical sequence, VNITIKQHTVTTTT, was also prepared (Fig. 1). Two peptides were chemically synthesized and purified on HPLC (purity, 99.20%, 99.10%, respectively) by Sigma Genosis Japan (Ishikari, Hokkaido).