Effects of genistein on secretion of extracellular matrix components and transforming growth factor beta in high-glucose-cultured rat mesangial cells
Effects of genistein on secretion of extracellular matrix components and transforming growth factor beta in high-glucose-cultured rat mesangial cells
复制标题
DOI：
10.1007/s10047-009-0479-y
复制
发表时间：
2009-12-01
影响因子：
1.3
通讯作者：
Meng, Jian-Zhong
中科院分区：
文献类型：
--
作者：
Yuan, Wei-Jie;Jia, Feng-Yu;Meng, Jian-Zhong
糖尿病肾病的理想治疗应减缓肾衰竭的进展，延迟透析的开始，并保护接受透析患者的残余肾功能。肾系膜细胞在这些过程中发挥重要作用。在本研究中，我们研究了染料木黄酮对高糖环境下培养的大鼠肾系膜细胞的影响。由于细胞外基质（ECM）成分（IV型胶原和纤连蛋白）和转化生长因子β（TGF-β）的过度表达先前已被牵连在糖尿病肾病肾小球损伤的发展，我们在我们的研究中包括这些物质。结果表明，高浓度葡萄糖（450 mg中心点dl（-1））刺激IV型胶原和纤维连接蛋白的合成和TGF-β的分泌。染料木黄酮（1份/千元5 μ mol中心点1（-1））以剂量和时间依赖性方式减弱这些反应。总之，我们证明，染料木黄酮可以抑制ECM成分的分泌和TGF-β的表达在蛋白和mRNA水平。这些发现应通过进一步的研究和临床试验来验证染料木黄酮对糖尿病肾病的潜在治疗作用。
The ideal treatment for diabetic nephropathy should slow the progress of renal failure, delay the initiation of dialysis, and protect residual renal function in patients receiving dialysis. Renal mesangial cells play an important role in these processes. In the current study, we investigated the effects of genistein on rodent renal mesangial cells cultured in a high-glucose environment. Since overexpression of the extracellular matrix (ECM) components (type IV collagen and fibronectin) and transforming growth factor beta (TGF-beta) have been previously implicated in the development of the renal glomerulus damage of diabetic nephropathy, we included these substances in our study. The results showed that high concentration of glucose (450 mg center dot dl(-1)) stimulated the synthesis of type IV collagen and fibronectin and the secretion of TGF-beta. These responses were attenuated by genistein (a parts per thousand yen5 mu mol center dot l(-1)) in a dose- and time-dependent manner. In conclusion, we demonstrated that genistein could inhibit the secretion of ECM components and the expression of TGF-beta at both the protein and mRNA levels. These findings should be followed up by further studies and clinical trials to verify the potential therapeutic effects of genistein on diabetic nephropathy.