Activation of adult-born neurons facilitates learning and memory
Activation of adult-born neurons facilitates learning and memory
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DOI：
10.1038/nn.3108
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发表时间：
2012-06-01
影响因子：
25
通讯作者：
Lledo, Pierre-Marie
中科院分区：
文献类型：
--
作者：
Alonso, Mariana;Lepousez, Gabriel;Lledo, Pierre-Marie
每天有数千个局部中间神经元到达成年啮齿动物的嗅球，但这一过程的功能效果仍然难以捉摸。通过在出生后的小鼠神经元中选择性表达通道视紫红质，我们发现它们的激活加速了困难的气味辨别学习并改善了记忆。这种改善被认为是同时发生的光活化与气味介绍，但不是当气味传递滞后500毫秒。此外，学习促进时，闪光灯在40 Hz，但不是在10 Hz。在体外和体内的二尖瓣细胞的电生理记录显示，40-Hz的刺激产生增强GABA能抑制相比，10-Hz的刺激。促进学习发生时，特别是在成年期产生的光激活神经元。综上所述，我们的研究结果表明，成人出生的神经元的活动和嗅球电路的功能之间存在直接的因果关系。
Thousand of local interneurons reach the olfactory bulb of adult rodents every day, but the functional effect of this process remains elusive. By selectively expressing channelrhodopsin in postnatal-born mouse neurons, we found that their activation accelerated difficult odor discrimination learning and improved memory. This amelioration was seen when photoactivation occurred simultaneously with odor presentation, but not when odor delivery lagged by 500 ms. In addition, learning was facilitated when light flashes were delivered at 40 Hz, but not at 10 Hz. Both in vitro and in vivo electrophysiological recordings of mitral cells revealed that 40-Hz stimuli produced enhanced GABAergic inhibition compared with 10-Hz stimulation. Facilitation of learning occurred specifically when photoactivated neurons were generated during adulthood. Taken together, our results demonstrate an immediate causal relationship between the activity of adult-born neurons and the function of the olfactory bulb circuit.