ARACHIDONIC-ACID METABOLIC PROFILES IN HUMAN MENINGIOMAS AND GLIOMAS
ARACHIDONIC-ACID METABOLIC PROFILES IN HUMAN MENINGIOMAS AND GLIOMAS
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DOI：
10.1007/bf00148394
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发表时间：
1987-01-01
影响因子：
3.9
通讯作者：
PAOLETTI, P
中科院分区：
文献类型：
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作者：
CASTELLI, MG;BUTTI, G;PAOLETTI, P
我们测定了人类颅内肿瘤（神经胶质瘤和脑膜瘤）样本以及正常脑组织样本中的花生四烯酸 (AA) 环加氧酶代谢谱。在手术中收集样本并立即冷冻在液氮中。组织匀浆对内源性 AA 进行离体代谢后，通过高分辨率气相色谱-质谱法测量 AA 的五种稳定代谢物（PGE2、PGD2、PGF2、α、6-酮基-PGF1.α 和 TXB2）。尽管脑膜瘤和神经胶质瘤表现出特征性特征，但样品之间 AA 代谢物的绝对量差异很大。与缓慢生长的良性脑膜瘤相比，快速生长的浸润性神经胶质瘤的 TXA2（据报道为促癌代谢物）合成较高，而 PGD2 和 PG12（据报道为抗癌代谢物）合成较低。在胶质母细胞瘤中发现比非病理性脑组织具有更高的总体合成能力，尤其是 TXA2。
We determined arachidonic acid (AA) cyclooxygenase metabolic profiles in specimens of human intracranial tumors (gliomas and meningiomas) and, when available, normal brain tissue. Samples were collected at surgery and immediately frozen in liquid nitrogen. The five stable metabolites of AA (PGE2, PGD2 PGF2,.alpha., 6-keto-PGF1.alpha. and TXB2) were measured by high-resolution gas chromatography-mass spectrometry after ex vivo metabolism of endogenous AA by tissue homogenates. The absolute amounts of AA metabolites varied widely between samples, though meningiomas and gliomas showed characteristic profiles. Compared to the slow-growing benign meningiomas, the rapidly-growing infiltrating gliomas had higher synthesis of TXA2 (reported as a procancer metabolite) and lower synthesis of PGD2 and PG12 (reported as anticancer metabolites). A higher overall synthesis capacity, preferentially toward TXA2, was found in glioblastomas than in non-pathological brain tissue.