Detection and identification of Wolbachia endosymbionts from laboratory stocks of stored-product insect pests and their parasitoids
Detection and identification of Wolbachia endosymbionts from laboratory stocks of stored-product insect pests and their parasitoids
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DOI：
10.1016/j.jspr.2009.07.003
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Miyanoshita, Akihiro
中科院分区：
文献类型：
--
作者：
Kageyama, Daisuke;Narita, Satoko;Miyanoshita, Akihiro
属于沃尔巴克氏体属的内共生细菌是母系遗传的，并且可以对其无脊椎动物宿主的生殖系统造成各种影响。由于Wolbachia对昆虫繁殖的重要影响，它们是未来害虫控制策略的潜在工具。在这里，我们探讨了沃尔巴克氏体感染的股票收藏在日本国家食品研究所，其中存储产品害虫和它们的寄生虫。应用PCR和序列分析方法，在鞘翅目、啮虫目、鳞翅目和膜翅目共38种59株Wolbachia中，有13种18株Wolbachia感染。根据wsp基因序列鉴定了14株Wolbachia。数据库检索显示，至少有6个沃尔巴克氏体菌株被多个宿主物种共享。在药房甲虫Stegobium paniceum和Angoumois谷物蛾Sitotroga cerealella中发现了两种不同的Wolbachia菌株的共感染。这些害虫及其寄生蜂的沃尔巴克氏体菌株的检测和表征为我们提供了未来的害虫控制策略，使用这些内共生细菌的基本信息。(C)2009爱思唯尔有限公司版权所有。
Endosymbiotic bacteria belonging to the genus Wolbachia are maternally inherited and can cause various effects on the reproductive system of their invertebrate hosts. Owing to their critical effects on insect reproduction, Wolbachia are potential tools for future insect pest control strategies. Here, we explored Wolbachia infections among stock collections at the National Food Research Institute of Japan, where stored-product insect pests and their parasitoids are maintained. Diagnostic polymerase chain reactions (PCRs) and sequencing of a fragment of the Wolbachia surface protein (wsp) gene revealed that, among 59 strains (38 species) belonging to the Coleoptera, Psocoptera, Lepidoptera and Hymenoptera, 18 strains (13 species) were infected with Wolbachia. Among them, 14 strains of Wolbachia were identified based on wsp gene sequences. Database searches showed that at least 6 Wolbachia strains were shared by multiple host species. Co-infections with two distinct Wolbachia strains were found in the drugstore beetle Stegobium paniceum and Angoumois grain moth Sitotroga cerealella. Detection and characterization of Wolbachia strains from these pests and their parasitoids provide us with basic information for future pest control strategies using these endosymbiotic bacteria. (C) 2009 Elsevier Ltd. All rights reserved.