ACETYLENE REDUCTION BY NITROGEN-FIXING PREPARATIONS FROM CLOSTRIDIUM PASTEURIANUM
ACETYLENE REDUCTION BY NITROGEN-FIXING PREPARATIONS FROM CLOSTRIDIUM PASTEURIANUM
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DOI：
10.1016/0304-4165(66)90383-7
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发表时间：
1966-01-01
期刊：
影响因子：
--
通讯作者：
DILWORTH, MJ
中科院分区：
文献类型：
--
作者：
DILWORTH, MJ
1.1.低分压乙炔抑制巴氏梭菌无细胞提取物的固氮作用。在氮气中生长的细胞的提取物与H2一起还原乙炔，但在NH4+中生长的细胞的类似提取物不能还原乙炔。产物经质谱学和红外光谱分析确证为乙烯。用H_2还原乙炔的要求与N_2还原--铁氧还蛋白、三磷酸腺苷和镁离子的要求相同。乙烯不能被萃取物进一步还原，并且不干扰氮气或乙炔的还原。当2H2O中的萃取物暴露在H_2和乙炔中时，产生了Purecis-[2H_2]-乙烯，这表明还原酶具有立体化学专一性。结果表明，乙炔还原和氮气还原是相似的过程，由相同的酶系统催化。结果表明，N_2还原的第一步是两个电子的还原，在二酰亚胺的氧化水平上生成不可分解的中间体。
1.1. Nitrogen fixation in cell-free extracts ofClostridium pasteurianumis inhibited by low partial pressures of acetylene.2.2. Extracts from cells grown on N2reduce acetylene with H2, but similar extracts from cells grown with NH4+do not. The product was characterized by mass spectrometry and infrared spectroscopy as ethylene.3.3. The requirements for acetylene reduction with H2are the same as those for N2reduction-ferredoxin, ATP and Mg2+.4.4. Ethylene cannot be further reduced by the extracts, and does not interfere with the reduction of either N2or acetylene.5.5. When extracts in2H2O were exposed to H2and acetylene, purecis-[2H2]-ethylene was produced, indicating stereochemical specificity in the reducing enzyme.6.6. The conclusion is drawn that acetylene and N2reduction are analogous processes, catalyzed by the same enzyme system. It is suggested that the first step in N2reduction is a two-electron reduction leading to a non-dissociable intermediate at the oxidation level of diimide.