The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.
The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.
复制标题
Mu转座子切除的晚期发育模式是由转基因玉米中花椰菜花叶病毒35S驱动的MURA cDNA赋予的。
DOI：
10.1105/tpc.12.1.5
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Walbot,V
中科院分区：
文献类型：
--
作者：
Raizada,MN;Walbot,V
TheMuDRelement responsible forMutatoractivities in maize encodes two genes,mudrAandmudrB.Each encodes multiple transcripts hypothesized to regulate, directly or indirectly, the unique late timing and switch in transposition mechanism during maize development.mudrA, which encodes the MURA transposase, is unstable in bacterial plasmids, a technical problem solved by using phage M13 as a vector to prepare DNA for biolistic transformation. In transgenic maize, a single 2.7-kbmudrAcDNA predicted to encode an 823–amino acid protein is sufficient to catalyze late somatic excisions, despite removal of the native promoter, alternative transcription start sites, known introns, polymorphic 5′ and 3′ untranslated sequences, and themudrBgene. These results suggest that post-translational regulation confersMuexcision timing. The transgene is active in lines containing silencingMuDRelements. This suggests that endogenousMuDRtransposons do not measurably immunize the host against expression of a homologous transgene.
