Transcriptional regulation of ABI3-and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis
Transcriptional regulation of ABI3-and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis
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DOI：
10.1007/s11103-005-2418-5
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发表时间：
2006-01-01
影响因子：
5.1
通讯作者：
Yamaguchi-Shinozaki, K
中科院分区：
文献类型：
--
作者：
Nakashima, K;Fujita, Y;Yamaguchi-Shinozaki, K
ABA响应元件（ABREs）是顺式作用元件，碱性亮氨酸拉链（bZIP）型ABRE结合蛋白（AREB）是响应脱落酸（阿坝）处理的拟南芥营养组织中RD 29 B表达的转录激活因子。脱水反应元件（DRES）作为ABRE的偶联元件在RD 29 A响应阿坝的表达中起作用。利用abi 3和abi 5突变体进行的表达分析表明，ABI 3和ABI 5在种子中RD 29 B的表达中起重要作用。碱基替换分析表明，两个ABRE功能强，一个ABRE与DRE耦合功能弱的RD 29 A在胚胎中的表达。在瞬时反式激活实验中，ABI 3、ABI 5和AREB 1强烈激活由RD 29 B启动子驱动的GUS报告基因的转录，但这些蛋白质微弱激活由RD 29 A启动子驱动的转录。在35 S：：ABI 3拟南芥中，RD 29 B的表达上调强烈，而RD 29 A的表达上调较弱。这些结果表明，在启动子中具有ABRE的RD 29 B的表达被ABI 3强烈上调，而在启动子中具有一个与DRE偶联的ABRE的RD 29 A的表达被ABI 3微弱上调。我们比较了7000拟南芥基因的表达响应阿坝处理在萌发过程中，在营养生长阶段，并在35 S：：ABI 3植物使用全长cDNA微阵列。ABI 3-和/或ABA-响应基因和顺式元件的启动子中的表达进行了讨论。
ABA-responsive elements (ABREs) are cis-acting elements and basic leucine zipper (bZIP)-type ABRE-binding proteins (AREBs) are transcriptional activators that function in the expression of RD29B in vegetative tissue of Arabidopsis in response to abscisic acid (ABA) treatment. Dehydration-responsive elements (DREs) function as coupling elements of ABRE in the expression of RD29A in response to ABA. Expression analysis using abi3 and abi5 mutants showed that ABI3 and ABI5 play important roles in the expression of RD29B in seeds. Base-substitution analysis showed that two ABREs function strongly and one ABRE coupled with DRE functions weakly in the expression of RD29A in embryos. In a transient transactivation experiment, ABI3, ABI5 and AREB1 activated transcription of a GUS reporter gene driven by the RD29B promoter strongly but these proteins activated the transcription driven by the RD29A promoter weakly. In 35S::ABI3 Arabidopsis plants, the expression of RD29B was up-regulated strongly, but that of RD29A was up-regulated weakly. These results indicate that the expression of RD29B having ABREs in the promoter is up-regulated strongly by ABI3, whereas that of RD29A having one ABRE coupled with DREs in the promoter is up-regulated weakly by ABI3. We compared the expression of 7000 Arabidopsis genes in response to ABA treatment during germination and in the vegetative growth stage, and that in 35S::ABI3 plants using a full-length cDNA microarray. The expression of ABI3- and/or ABA-responsive genes and cis-elements in the promoters are discussed.