ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells.
ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells.
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DOI：
10.1083/jcb.200202025
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发表时间：
2002-07-22
期刊：
影响因子：
--
通讯作者：
Burnstock G
中科院分区：
文献类型：
--
作者：
Ryten M;Dunn PM;Neary JT;Burnstock G
ATP作为细胞内能量来源的作用是众所周知的。然而，越来越多的人意识到它作为细胞外信使分子的作用。虽然骨骼肌成肌细胞上存在细胞外ATP受体的证据首次发表于1983年（Kolb和Wakelam），但其生理功能仍不清楚。本研究利用原代培养的大鼠骨骼肌卫星细胞研究嘌呤能信号在肌肉形成中的作用。使用免疫细胞化学，RT-PCR和电生理学，我们证明，离子型P2 X5受体是目前卫星细胞和P2 X受体的激活抑制增殖，刺激表达的标志物的肌细胞分化，包括肌细胞生成素，p21和肌球蛋白重链，并增加肌管形成的速度。此外，我们证明，ATP的应用结果在一个显着的和快速增加的磷酸化的MAPK，特别是p38，和抑制p38活性可以防止ATP对细胞数量的影响。这些结果不仅证明了骨骼肌分化的一种新的调节剂，即ATP的存在，而且还证明了离子型P2 X受体在控制细胞命运中的新作用。
ATP is well known for its role as an intracellular energy source. However, there is increasing awareness of its role as an extracellular messenger molecule. Although evidence for the presence of receptors for extracellular ATP on skeletal myoblasts was first published in 1983 (Kolb and Wakelam), their physiological function has remained unclear. In this paper we used primary cultures of rat skeletal muscle satellite cells to investigate the role of purinergic signaling in muscle formation. Using immunocytochemistry, RT-PCR, and electrophysiology, we demonstrate that the ionotropic P2X5 receptor is present on satellite cells and that activation of a P2X receptor inhibits proliferation, stimulates expression of markers of muscle cell differentiation, including myogenin, p21, and myosin heavy chain, and increases the rate of myotube formation. Furthermore, we demonstrate that ATP application results in a significant and rapid increase in the phosphorylation of MAPKs, particularly p38, and that inhibition of p38 activity can prevent the effect of ATP on cell number. These results not only demonstrate the existence of a novel regulator of skeletal muscle differentiation, namely ATP, but also a new role for ionotropic P2X receptors in the control of cell fate.
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DOI：
10.1006/bbrc.2000.3486
发表时间：
2000-09-24
影响因子：
3.1
作者：
Chun, YK;Kim, J;Kim, SS
通讯作者：
Kim, SS
影响因子：
3.5
作者：
Ferrari, D;Los, M;Schulze-Osthoff, K
通讯作者：
Schulze-Osthoff, K
影响因子：
4.8
作者：
Favata, MF;Horiuchi, KY;Trzaskos, JM
通讯作者：
Trzaskos, JM
影响因子：
4.8
作者：
ALESSI, DR;CUENDA, A;SALTIEL, AR
通讯作者：
SALTIEL, AR
影响因子：
64.8
作者：
KOLB, HA;WAKELAM, MJO
通讯作者：
WAKELAM, MJO