Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy
Micro-computed tomographic analysis of the radial geometry of intrarenal artery-vein pairs in rats and rabbits: Comparison with light microscopy
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DOI：
10.1111/1440-1681.12842
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发表时间：
2017-12-01
影响因子：
2.9
通讯作者：
Evans, Roger G.
中科院分区：
文献类型：
--
作者：
Ngo, Jennifer P.;Le, Bianca;Evans, Roger G.
我们评估了同步辐射显微计算机断层扫描（micro-CT）用于定量肾皮质血管径向几何形状的实用性。将9只大鼠和6只家兔的肾脏灌注固定，并用Microfil填充肾循环。为了评估Microfil的收缩，在组织制备后立即在Australian Synchrotron对大鼠肾脏进行成像，然后在多聚甲醛中进行后固定，并在24小时后重新成像。Microfil在24小时内仅收缩了2-5%。所有后续微型CT成像均在样本制备后24小时内完成。在显微CT成像后，对肾脏进行组织学分析。在大鼠和兔肾脏中，在组织学切片中识别的血管结构可以在来自原始肾脏的二维（2D）微CT图像中识别。两组图像中的血管形态相似。通过手动分析micro-CT的2D图像量化的径向几何形状与光学显微镜产生的相应数据一致。然而，由于使用对比度增强的微型CT对整个器官成像时空间分辨率有限，只能分别评估大鼠和兔直径>= 100 μ m和>= 60 μ m的动脉。我们的结论是，它是可行的和有效的，使用micro-CT定量血管几何形状的肾皮质循环在大鼠和家兔。然而，需要结合光学显微镜和显微CT方法来评估在广泛的血管尺寸范围内肾内动脉和静脉之间的空间关系。
We assessed the utility of synchrotron-radiation micro-computed tomography (micro-CT) for quantification of the radial geometry of the renal cortical vasculature. The kidneys of nine rats and six rabbits were perfusion fixed and the renal circulation filled with Microfil. In order to assess shrinkage of Microfil, rat kidneys were imaged at the Australian Synchrotron immediately upon tissue preparation and then post fixed in paraformaldehyde and reimaged 24hours later. The Microfil shrank only 2-5% over the 24hour period. All subsequent micro-CT imaging was completed within 24hours of sample preparation. After micro-CT imaging, the kidneys were processed for histological analysis. In both rat and rabbit kidneys, vascular structures identified in histological sections could be identified in two-dimensional (2D) micro-CT images from the original kidney. Vascular morphology was similar in the two sets of images. Radial geometry quantified by manual analysis of 2D images from micro-CT was consistent with corresponding data generated by light microscopy. However, due to limited spatial resolution when imaging a whole organ using contrast-enhanced micro-CT, only arteries >= 100 and >= 60 mu m in diameter, for the rat and rabbit respectively, could be assessed. We conclude that it is feasible and valid to use micro-CT to quantify vascular geometry of the renal cortical circulation in both the rat and rabbit. However, a combination of light microscopic and micro-CT approaches are required to evaluate the spatial relationships between intrarenal arteries and veins over an extensive range of vessel size.