Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells
Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells
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DOI：
10.1021/jf030526d
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发表时间：
2004-05-19
影响因子：
6.1
通讯作者：
Friedman, M
中科院分区：
文献类型：
--
作者：
Lee, KR;Kozukue, N;Friedman, M
作为改善植物源性食品（如马铃薯、茄子和番茄）的努力的一部分，使用微量培养四唑（MTT）测定法检查了一系列结构相关的单个化合物对人结肠癌（HT 29）和肝癌（HepG 2）癌细胞的抗增殖活性。目的是评估的作用，影响这些化合物的抑制活性的茄苷的糖侧链和苷元部分。评价用四种浓度进行，（0.1、1、10和100 μ g/mL）的马铃薯三糖配糖生物碱α-查康宁和α-龙葵碱;二糖β（1）-查康宁、β（2）-查康宁和β（2）-龙葵碱;单糖γ-查康宁和它们共同的糖苷配基龙葵定;四糖马铃薯配糖生物碱去氢异甘草酸;马铃薯糖苷配基去甲基异甘草酸;四糖番茄配糖生物碱α-番茄碱、三糖β（1）-番茄碱、二糖γ-番茄碱、单糖δ-番茄碱和它们共同的配糖基番茄碱;茄子配糖生物碱茄边碱和茄碱和它们共同的配糖基茄碱;和非甾体生物碱jervine。所有化合物在测定中均具有活性，其中配糖生物碱的活性最高，水解产物的活性较低。对肝细胞的有效性大于对结肠细胞的有效性。浓度为1 μ g/mL的α-番茄碱和α-查康宁对肝癌细胞的效力高于用抗癌药物阿霉素和喜树碱观察到的效力。由于α-查乌头碱、α-龙葵碱和α-番茄碱也抑制正常人肝HeLa（Chang）细胞，因此安全性考虑应指导这些化合物作为癌症的预防或治疗性治疗的使用。
As part of an effort to improve plant-derived foods such as potatoes, eggplants, and tomatoes, the antiproliferative activities against human colon (HT29) and liver (HepG2) cancer cells of a series of structurally related individual compounds were examined using a microculture tetrazolium (MTT) assay. The objective was to assess the roles of the carbohydrate side chain and aglycon part of Solanum glycosides in influencing inhibitory activities of these compounds. Evaluations were carried out with four concentrations each (0.1, 1, 10, and 100,mug/mL) of the the potato trisaccharide glycoalkaloids alpha-chaconine and alpha-solanine; the disaccharides beta(1)-chaconine, beta(2)-chaconine, and beta(2)-solanine; the monosaccharide gamma-chaconine and their common aglycon solanidine; the tetrasaccharide potato glycoalkaloid dehydrocommersonine; the potato aglycon demissidine; the tetrasaccharide tomato glycoalkaloid alpha-tomatine, the trisaccharide beta(1)-tomatine, the disaccharide gamma-tomatine, the monosaccharide delta-tomatine, and their common aglycon tomatidine; the eggplant glycoalkaloids solamargine and solasonine and their common aglycon solasodine; and the nonsteroidal alkaloid jervine. All compounds were active in the assay, with the glycoalkaloids being the most active and the hydrolysis products less so. The effectiveness against the liver cells was greater than against the colon cells. Potencies of alpha-tomatine and alpha-chaconine at a concentration of 1 mug/mL against the liver carcinoma cells were higher than those observed with the anticancer drugs doxorubicin and camptothecin. Because alpha-chaconine, alpha-solanine, and alpha-tomatine also inhibited normal human liver HeLa (Chang) cells, safety considerations should guide the use of these compounds as preventative or therapeutic treatments against carcinomas.