Milk quality parameters associated with the occurrence of veterinary drug residues in bulk tank milk
Milk quality parameters associated with the occurrence of veterinary drug residues in bulk tank milk
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DOI：
10.1590/1678-992x-2016-0120
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发表时间：
2017-06-01
影响因子：
2.6
通讯作者：
Bordignon-Luiz, Marilde Terezinha
中科院分区：
文献类型：
--
作者：
Picinin, Lidia Cristina Almeida;Toaldo, Isabela Maia;Bordignon-Luiz, Marilde Terezinha
散装罐装牛奶中的兽药残留对乳品链的所有环节都很重要，因为它们是决定最终产品安全性的主要因素之一。本研究试图使用多元主成分分析(PCA)来确定与兽药残留发生相关的牛奶质量参数。从巴西米纳斯吉拉斯州45个奶牛场采集了132份原料奶样品，采用串联模式的高效液相色谱-质谱仪和气相色谱-电子捕获检测法分析了包括拟除虫菊酯、大环内酯类和抗菌剂在内的42种分析物质。在132个牛奶样本中，有40个样本检测出至少一种分析物呈阳性(超过检测限值)。通过确认试验，与抗菌剂残留相关的牛奶参数是乳糖和脱脂浓度，如PCA所揭示的那样。这一分析表明，散装罐装牛奶中的脂肪和总固体浓度，以及体细胞和细菌总数与大环内酯残留量有关。对散装罐装牛奶中拟除虫菊酯残留量的主成分分析表明，乳糖和脱脂固体浓度以及可滴定的酸度与这些残留量呈负相关。因此，数据分析表明，兽药残留与某些牛奶质量参数有关，这些参数可用于针对兽药残留污染风险较高的牧场进行检测计划，并结合激励、教育和培训计划，以改善乳房健康、牛奶卫生和安全。
Veterinary drug residues in bulk tank milk are important to all sectors of the dairy chain because they are one of the major factors which determine the safety of the final product. This study attempted to identify milk quality parameters that are associated with the occurrence of veterinary drug residues using multivariate principal component analysis (PCA). A total of 132 raw milk samples were collected from 45 dairy farms in the state of Minas Gerais-Brazil and analyzed for 42 analytes, including pyrethroids, macrocyclic lactones and antibacterials, using liquid chromatography coupled with mass spectrometry in tandem mode and gas chromatography with electron capture detection. Out of the 132 milk samples, 40 samples tested positive for at least one analyte (above the detection limit). The milk parameters associated with the antimicrobial residues by confirmatory tests were lactose and nonfat concentrations, as revealed by PCA. This analysis showed that fat and total solid concentrations, as well as the somatic cell and total bacteria counts were associated with macrocyclic lactone residues in bulk tank milk. A PCA assessing pyrethroid residues in bulk tank milk revealed that the lactose and nonfat solid concentrations and titratable acidity were inversely associated with these residues. Thus, the data analysis indicated that the veterinary drug residues were associated with certain milk quality parameters that can be used to target farms at higher risk of veterinary drug residue contamination for testing programs in combination with incentives, education and training programs to improve mammary health, milk hygiene and safety.