Profiling circular RNA in methamphetamine-treated primary cortical neurons identified novel circRNAs related to methamphetamine addiction
Profiling circular RNA in methamphetamine-treated primary cortical neurons identified novel circRNAs related to methamphetamine addiction
复制标题
对甲基苯丙胺处理的初级皮层神经元中的环状 RNA 进行分析，发现与甲基苯丙胺成瘾相关的新型 circRNA
DOI：
10.1016/j.neulet.2019.02.032
复制
发表时间：
2019-05-14
影响因子：
2.5
通讯作者：
Sun, Jinhao
中科院分区：
文献类型：
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作者：
Li, Junwei;Shi, Qingqing;Sun, Jinhao
甲基苯丙胺（METH）多年来一直是全球健康威胁。近年来的研究发现，环状RNA（circular RNA，circRNA）在脑组织中的表达量非常丰富，且呈动态变化。然而，circRNA和甲基苯丙胺诱导的神经毒性之间的联系仍然不确定。在本研究中，原代皮层神经元在体外用METH处理。我们通过高通量RNA测序分析了circRNA，并鉴定了2458个circRNA。进行生物信息学分析以预测这些circRNA的潜在功能，其揭示了可能有助于METH引起的神经元损伤的发病机制的几个相关途径，包括“吗啡成瘾”。特别地，使用条件位置偏好范式建立了一个METH成瘾小鼠模型，用于验证筛选的circRNA。最后，我们建立了circRNA与miRNA和mRNA的共表达网络，以展示它们之间的潜在关联。总之，我们首次揭示了circRNA在METH诱导的神经元损伤和METH成瘾中的作用。
Methamphetamine (METH) has been a worldwide health threat for years. Recent studies have reported that circular RNA (circRNA) are highly abundant and dynamically expressed in brain. However, connections between circRNA and METH-induced neurotoxicity remains indefinite. In the present study, primary cortical neurons were treated with METH in vitro. We profiled circRNA via high-throughput RNA sequencing and identified 2458 circRNAs. Bioinformatics analysis was performed to predict potential functions of these circRNAs which revealed several relevant pathways including 'morphine addiction' that may contribute to the pathogenesis of neuronal damage by METH. Especially, a METH-addicted mouse model was established with conditional place preference paradigm for validation of screened circRNAs. At last, we established co-expression networks of circRNAs with miRNAs and mRNAs to exhibit potential association among them. In conclusion, we firstly unveiled a role of circRNAs in METH-induced neuronal damage and METH addiction.