Enterocyte and M-cell transport of native and heat-denatured bovine beta-lactoglobulin: significance of heat denaturation.
Enterocyte and M-cell transport of native and heat-denatured bovine beta-lactoglobulin: significance of heat denaturation.
复制标题
天然和热变性牛 β-乳球蛋白的肠上皮细胞和 M 细胞转运：热变性的意义。
DOI：
10.1021/jf052309d
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发表时间：
2006
影响因子：
6.1
通讯作者：
T. Karttunen
中科院分区：
文献类型：
--
作者：
J. Rytkönen;K. Valkonen;V. Virtanen;R. Foxwell;J. Kyd;A. Cripps;T. Karttunen
牛 β-乳球蛋白 (β-Ig) 的三维结构、消化率和免疫特性通过液态奶制品加工中的热处理进行了改变。由于尚不清楚此类治疗是否也会改变 β-Ig 的肠道转运特性，因此使用 Caco-2 细胞和 M 细胞在实验细胞模型中研究了天然牛 β-Ig 和热变性牛 β-Ig 的转运。用荧光标记物标记的 β-Ig 的转运随后进行荧光测量、电泳分析和荧光显微镜检查。数据显示，这两种细胞类型运输天然 β-Ig 的效率比运输热变性 β-Ig 的效率更高。此外，M 细胞比 Caco-2 细胞转运更多的天然 β-Ig。天然和热变性的β-Ig 的运输是跨细胞的。电泳数据还表明，热变性的 β-Ig 在运输过程中可能比天然 β-Ig 降解得更多。
The three-dimensional structure, digestibility, and immunological properties of bovine beta-lactoglobulin (beta-lg) are modified by heat treatments used in processing of liquid milk products. Because it is not known if such treatments also modify the intestinal transport properties of beta-lg, the transport of native and heat-denatured bovine beta-lg was investigated in experimental cell models using Caco-2 cells and M cells. Transport of beta-lg labeled with a fluorescent marker was followed with fluorometric measurements, electrophoretic analyses, and fluorescence microscopy. The data show that both cell types transported native beta-lg more efficiently than they did heat-denatured beta-lg. In addition, M cells transported native beta-lg more than Caco-2 cells. Transport of native and heat-denatured beta-lg was transcellular. The electrophoretic data also suggest that heat-denatured beta-lg may have degraded more than native beta-lg during the transport.