Rap2a Is Negatively Regulated by NF-κB and Contributes to Growth via Wnt Pathway in Shrimp
Rap2a Is Negatively Regulated by NF-κB and Contributes to Growth via Wnt Pathway in Shrimp
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DOI：
10.3389/fmars.2022.942009
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发表时间：
2022-07
期刊：
影响因子：
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通讯作者：
Sheng Wang;Mingyu He;Qinyao Li;Haoyang Li;Bin Yin;Jianguo He;Chaozheng Li
中科院分区：
文献类型：
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作者：
Sheng Wang;Mingyu He;Qinyao Li;Haoyang Li;Bin Yin;Jianguo He;Chaozheng Li
Rap 2a是Ras家族小GTP酶的一员，在生长发育中起着关键作用。到目前为止，还没有在甲壳类动物中发现Rap 2a同源物。本研究克隆并鉴定了凡纳滨对虾Rap 2a的一个同源基因，命名为LvRap 2a。LvRap 2a与来自不同物种的其他Rap 2a同源物相比具有较高的序列保守性。LvRap 2a在血细胞、肌肉、肝胰腺和肠组织中大量表达。重要的是，Rap 2a敲低在生长实验中有效地抑制了虾的长度和重量。从机制上讲，我们发现Rap 2a可以诱导几种Wnt的表达，这些Wnt是细胞生长、分化和组织稳态的正调节因子。LvRap 2a的表达受天然免疫信号通路转录因子LvDorsal（NF-κB）负调控。这些结果表明，LvRap 2a可能通过激活Wnt信号通路促进对虾的生长，LvRap 2a可能是天然免疫与生长之间的“串话”，LvRap 2a可作为对虾促生长的潜在靶点。
Rap2a, a member of Ras family small GTPases, plays a key role in growth and development. So far, no Rap2a homolog has been discovered in crustaceans. Herein, a Rap2a homolog from shrimp Litopenaeus vannamei, named LvRap2a, was cloned and identified. LvRap2a showed a high level of sequence conservation to other Rap2a homologs from different species. LvRap2a was found to be abundantly expressed in hemocytes, muscle, hepatopancreas and intestine tissues. Importantly, Rap2a knockdown effectively suppressed the length and weight of shrimp in a growth experiment. Mechanistically, we found that Rap2a could induce the expression of several Wnts, which are the positive regulators in cellular growth, differentiation, and tissue homeostasis. Moreover, LvRap2a expression was observed to be negatively regulated by LvDorsal (NF-κB), a transcription factor of innate immune signaling pathway. These results indicated that LvRap2a contributed to shrimp growth probably by activating the Wnt signaling pathway, and that LvRap2a could be the crosstalk between innate immunity and growth, as well as that LvRap2a could serve as a potential target for shrimp growth promotion.