Plasma treatment effects on destruction and recovery of Porphyromonas gingivalis biofilms.
Plasma treatment effects on destruction and recovery of Porphyromonas gingivalis biofilms.
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DOI：
10.1371/journal.pone.0274523
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
本研究的目的是研究非热大气气体等离子体 (NTAP) 对生物膜中牙龈卟啉单胞菌 (P. gingivalis) 的破坏和恢复（或重新定植）的处理效果。牙龈卟啉单胞菌是一种众所周知的关键牙周病原体，与牙周疾病，特别是牙周炎密切相关。在不锈钢试片上形成牙龈卟啉单胞菌生物膜，并用纯氩气和氩+氧气混合物的 NTAP 处理 1、2 和 5 分钟。使用MTT测定、菌落形成单位（CFU）计数测定和共焦激光扫描显微镜（CLSM）来评估破坏效率。此外，将等离子体处理的生物膜在补充有抗生素和氧化应激源的培养基中重新培养，以确定 NTAP 与其他抗菌剂的协同作用。结果表明，等离子体处理可使细菌负荷减少 2.7 个对数单位。用 NTAP 处理联合阿莫西林的亚最小抑制浓度恢复的生物膜 CFU 比单独用阿莫西林处理的生物膜少 0.33 个对数单位。 NTAP 组中恢复的生物膜 CFU 比 H2O2 处理下未处理的对照少约 2.0 个对数单位。在百草枯处理下，与未处理的对照相比，等离子体处理的生物膜中的生物膜 CFU 减少了约 1.0 个对数单位。等离子体处理的生物膜对阿莫西林的耐药性较低，对过氧化氢 (H2O2) 和百草枯的敏感性较高，表明 NTAP 可能增强生物膜对宿主防御的敏感性。这些体外研究结果表明 NTAP 可能是一种新颖有效的治疗引起牙周疾病的口腔生物膜的方法。
The objective of this study was to investigate the treatment effects of non-thermal atmospheric gas plasmas (NTAP) on destruction and the recovery (or re-colonization) of Porphyromonas gingivalis (P. gingivalis) in biofilms. P. gingivalis is a well-known keystone periodontal pathogen strongly associated with periodontal diseases, especially periodontitis. P. gingivalis biofilms were formed on stainless steel coupons and treated for 1, 2, and 5 minutes by NTAP of pure argon gas and argon+oxygen gas mixture. MTT assay, colony forming unit (CFU) counting assay and confocal laser scanning microscopy (CLSM) were used to assess the destruction efficiency. In addition, the plasma treated biofilms were re-cultured in the medium supplemented with antibiotics and oxidative stress sources to determine the synergy of the NTAP with other antimicrobial agents. The results showed the plasma treatment could result in 2.7 log unit reduction in bacterial load. The recovered biofilm CFU with NTAP treatment combined with sub minimal inhibition concentration of amoxicillin was 0.33 log units less than the biofilm treated with amoxicillin alone. The recovered biofilm CFU in NTAP groups was about 2.0 log units less than that in the untreated controls under H2O2 treatment. There was approximately 1.0 log unit reduction of biofilm CFU in plasma treated biofilm compared with untreated control under paraquat treatment. The plasma treated biofilms exhibited less resistance to amoxicillin and greater susceptibility to hydrogen peroxide (H2O2) and paraquat, suggesting that NTAP may enhance biofilm susceptibility to host defense. These in vitro findings suggested that NTAP could be a novel and effective treatment method of oral biofilms that cause periodontal diseases.