SEGMENTATION IN THE CHICK-EMBRYO HINDBRAIN IS DEFINED BY CELL LINEAGE RESTRICTIONS
SEGMENTATION IN THE CHICK-EMBRYO HINDBRAIN IS DEFINED BY CELL LINEAGE RESTRICTIONS
复制标题
DOI：
10.1038/344431a0
复制
发表时间：
1990-03-29
期刊：
影响因子：
64.8
通讯作者：
LUMSDEN, A
中科院分区：
文献类型：
--
作者：
FRASER, S;KEYNES, R;LUMSDEN, A
在鸡胚后脑，形态分割成rhormbomers匹配的早期神经元分化和轴突发生的同色异谱模式1。菱形之间的边界与鼠调节基因的表达边界一致2 -4。通过使用细胞内标记的克隆分析，我们在这里表明，菱形边界是细胞不移动的分区。当亲本细胞在菱形区边界出现之前被标记时，所产生的克隆能够扩散到相邻的菱形区中。当边界出现后标记时，克隆仍然在起源的菱形节内自由扩张，但它现在被限制在边界处。因此，鸡胚中的菱形体表现得像多克隆单位，这就增加了它们类似于昆虫的区室的可能性。
IN the chick embryo hindbrain, morphological segmentation into rhormbomeres is matched by metameric patterns of early neuronal differentiation and axonogenesis1. Boundaries between rhombomeres coincide with boundaries of expression of murine regulatory genes2–4. By clonal analysis using intracellular marking, we show here that the rhombomere boundaries are partitions across which cells do not move. When a parent cell is marked before the appearance of rhombomere boundaries, the resulting clone is able to spread into the neighbouring rhombomere. When marked after boundary appearance, the clone still expands freely within the rhombomere of origin, but it is now restricted at the boundaries. Rhombomeres in the chick embryo thus behave like polyclonal units, raising the possibility that they are analogous to the compart-ments of insects.