Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52
Characterisation of a new stripe rust resistance gene Yr47 and its genetic association with the leaf rust resistance gene Lr52
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DOI：
10.1007/s00122-011-1545-4
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Bariana, H. S.
中科院分区：
文献类型：
--
作者：
Bansal, U. K.;Forrest, K. L.;Bariana, H. S.
来自Watkins收集的两个伊朗普通小麦地方品种AUS 28183和AUS 28187显示出对澳大利亚叶锈病和条锈病病原体致病型的高水平的幼苗抗性。对AUS 28183和AUS 28187与感病基因型AUS 27229杂交的F3群体的锈病反应分离进行卡方分析，结果表明叶锈病和条锈病抗性均为单基因遗传。由于两种基因型产生相似的叶锈病和条锈病感染类型，因此假定它们携带相同的基因。这些基因暂时被命名为LrW 1和YrW 1。分子图谱将LrW 1和YrW 1定位在染色体5 B的短臂上，分别距离SSR标记gwm 234约10和15 cM，标记cfb 309定位在YrW 1附近8-12 cM。在两个F-3群体中，LrW 1定位在YrW 1远端3-6 cM处。AUS 28183对应于沃特金斯收藏的登录号V336，该收藏号是Lr 52的原始来源。基于基因组定位和登录记录，LrW 1被推断为Lr 52。由于没有其他的苗期条锈病抗性基因被定位在染色体5 BS上，因此YrW 1被永久命名为Yr 47。侧翼标记gwm 234和cfb 309与表型测定的组合可用于确定分离群体中Lr 52和Yr 47的存在。这项调查的特点是一个有价值的来源，双重叶锈病和条锈病抗性部署在新的小麦品种。将Lr 52和Yr 47转入当前澳大利亚小麦背景的工作正在进行中。
Two Iranian common wheat landraces AUS28183 and AUS28187 from the Watkins collection showed high levels of seedling resistance against Australian pathotypes of leaf rust and stripe rust pathogens. Chi-squared analyses of rust response segregation among F-3 populations derived from crosses of AUS28183 and AUS28187 with a susceptible genotype AUS27229 revealed monogenic inheritance of leaf rust and stripe rust resistance. As both genotypes produced similar leaf rust and stripe rust infection types, they were assumed to carry the same genes. The genes were temporarily named as LrW1 and YrW1. Molecular mapping placed LrW1 and YrW1 in the short arm of chromosome 5B, about 10 and 15 cM proximal to the SSR marker gwm234, respectively, and the marker cfb309 mapped 8-12 cM proximal to YrW1. LrW1 mapped 3-6 cM distal to YrW1 in two F-3 populations. AUS28183 corresponded to the accession V336 of the Watkins collection which was the original source of Lr52. Based on the genomic location and accession records, LrW1 was concluded to be Lr52. Because no other seedling stripe rust resistance gene has previously been mapped in chromosome 5BS, YrW1 was permanently named as Yr47. A combination of flanking markers gwm234 and cfb309 with phenotypic assays could be used to ascertain the presence of Lr52 and Yr47 in segregating populations. This investigation characterised a valuable source of dual leaf rust and stripe rust resistance for deployment in new wheat cultivars. Transfer of Lr52 and Yr47 into current Australian wheat backgrounds is in progress.