Induced over-expression of the transcription factor OsDREB2A improves drought tolerance in rice
Induced over-expression of the transcription factor OsDREB2A improves drought tolerance in rice
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诱导转录因子 OsDREB2A 过度表达可提高水稻的耐旱性
DOI：
10.1016/j.plaphy.2011.09.012
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发表时间：
2011-12-01
影响因子：
6.5
通讯作者：
Wu, Ray
中科院分区：
文献类型：
--
作者：
Cui, Meng;Zhang, Wenjiao;Wu, Ray
DREB和CBF转录因子在植物发育和非生物胁迫反应中起着关键作用，因此，它们是植物分子育种的重要目标。为了提高粳稻品种TNG67的非生物胁迫抗性，本研究克隆了水稻OsDREB2A基因，并在胁迫诱导型启动子（4ABRC）的调控下进行表达。发现过表达OsDREB2A的T（2）和T（3）转基因株系在严重干旱和盐胁迫条件下相对于非转基因水稻植株或用空载体对照转化的水稻植株具有提高的存活率。干旱和阿坝处理显著诱导OsDREB2A的表达。结果表明，在水稻工程植株中，由4ABRC启动子驱动的OsDREB2A的诱导过表达可能在胁迫期间保护细胞。(C)2011年Elsevier Masson SAS。All rights reserved.
The DREB and CBF transcription factors play a critical role in plant development and abiotic stress responses and, therefore, represent attractive targets for a molecular plant breeding approach. In this study, the rice OsDREB2A gene was isolated and expressed under the control of a stress-inducible promoter (4ABRC) to improve the abiotic stress tolerance of japonica rice variety TNG67. T(2) and T(3) transgenic lines over-expressing OsDREB2A were found to have improved survival rates under severe drought and salt stress conditions relative to non-transgenic rice plants or rice plants transformed with the empty vector control. OsDREB2A expression was found to be markedly induced by drought and ABA treatment. The results indicate that the induced over-expression of OsDREB2A driven by the 4ABRC promoter in engineered rice plants may protect cells during stress. (C) 2011 Elsevier Masson SAS. All rights reserved.