Synergetic effects of nanoporous support and urea on enzyme activity
Synergetic effects of nanoporous support and urea on enzyme activity
复制标题
DOI：
10.1021/nl070255b
复制
发表时间：
2007-04-01
期刊：
影响因子：
10.8
通讯作者：
Ackerman, Eric J.
中科院分区：
文献类型：
--
作者：
Lei, Chenghong;Shin, Yongsoon;Ackerman, Eric J.
我们报道了功能化介孔二氧化硅(FMS)和尿素的协同效应，以促进有利的蛋白质构象变化。包埋在有尿素的FMS中的葡萄糖异构酶(GI)的比活力约为无尿素溶液中的GI的两倍。在8.0M尿素的变性溶液中，包埋在FMS中的GI的比活性仍然高于溶液中GI的最高比活性，而不是失去所有的活性。
We report synergetic effects of functionalized mesoporous silica (FMS) and urea to promote favorable protein conformational changes. The specific activity of glucose isomerase (GI) entrapped in FMS in the presence of urea was approximately double that of GI in solution in the absence of urea. Rather than losing all activity in a denaturing solution of 8.0 M urea, the specific activity of GI entrapped in FMS remained higher than the highest specific activity of GI free in solution.