A rapid quantitative determination of acetaminophen in plasma.
A rapid quantitative determination of acetaminophen in plasma.
复制标题
血浆中对乙酰氨基酚的快速定量测定。
DOI：
--
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
F. Zurzola
中科院分区：
文献类型：
--
作者：
S. O'Connell;F. Zurzola
描述了一种快速定量分析血浆中对乙酰氨基酚的简单方法。给药后血浆中存在的非结合对乙酰氨基酚在血浆蛋白沉淀后通过高压液相色谱 (HPLC) 在 240 nm 波长下测定。对乙酰氨基酚 (I) 的检测浓度低至 0.1 微克/毫升。浓度范围为 0 至 32 微克/毫升的血浆标准品的平均回收率为 94%，变异系数为 3%。标准曲线斜率的测定间变异性的变异系数为 2.7%。描述了通过与在几个人体生物利用度研究期间同时运行的另一个程序进行比较来应用和验证该方法。
A simple method is described for the rapid, quantitative analysis of acetaminophen in plasma. The nonconjugated acetaminophen present in the plasma following drug administration is determined after plasma protein precipitation by high-pressure liquid chromatography (HPLC) at a wavelength of 240 nm. Acetaminophen (I) is detectable at levels as low as 0.1 microgram/ml. Mean recoveries of 94% with a coefficient of variation of 3% were obtained for plasma standards whose concentrations ranged from 0 to 32 microgram/ml. Interassay variability of the slope of the standard curve had a coefficient of variation of 2.7%. Application and verification of this method by comparison with another procedure run simultaneously during several human bioavailability studies are described.