Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae
Storage and secretion of Ag-Aper14, a novel peritrophic matrix protein, and Ag-Muc1 from the mosquito Anopheles gambiae
复制标题
DOI：
10.1007/s00441-004-1067-3
复制
发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Jacobs-Lorena, M
中科院分区：
文献类型：
--
作者：
Devenport, M;Fujioka, H;Jacobs-Lorena, M
Ag-Aper14基因在冈比亚按蚊中编码一种新的营养基质（或营养膜；PM）蛋白。Ag-Aper14蛋白只有89个氨基酸长，并且有一个假定的几丁质结合结构域。在供血之前，AgAper14蛋白被储存在上皮细胞管腔表面附近的分泌囊泡中。免疫电镜显示Ag-Aper14与另一种PM蛋白Ag-Aper1共定位于相同的分泌囊泡，表明一种共同的调节分泌模式。相反，上皮细胞表面蛋白Ag-Muc1不与这些分泌囊泡共定位，仅在细胞表面检测到。在供血后，Ag-Aper14被分泌并并入被摄入血液周围的PM中。
The gene Ag-Aper14 encodes a novel peritrophic matrix (or peritrophic membrane; PM) protein in the mosquito Anopheles gambiae. The Ag-Aper14 protein is merely 89 amino acids long and has a single putative chitin-binding domain. Prior to blood feeding, the AgAper14 protein is stored in secretory vesicles next to the epithelial cell lumenal surface. Immunoelectron microscopy has revealed that Ag-Aper14 co-localizes to the same secretory vesicles as another PM protein, Ag-Aper1, indicating a common mode of regulated secretion. Conversely, Ag-Muc1, an epithelial cell-surface protein, does not colocalize to these secretory vesicles and is detected only on the cell surface. After blood feeding, Ag-Aper14 is secreted and incorporated into the PM that surrounds the ingested blood.