Expression of the monocarboxylate transporter MCT2 by rat brain glia
Expression of the monocarboxylate transporter MCT2 by rat brain glia
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DOI：
10.1002/(sici)1098-1136(199803)22:3
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发表时间：
1998-03
期刊：
影响因子：
6.2
通讯作者：
D. Z. Gerhart;Bradley E. Enerson;Olga Zhdankina;R. Leino;L. Drewes
中科院分区：
文献类型：
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作者：
D. Z. Gerhart;Bradley E. Enerson;Olga Zhdankina;R. Leino;L. Drewes
大鼠单羧酸转运蛋白MCT2的核苷酸序列是从脑组织中提取的。在鸡中制备了针对推导的氨基酸序列的羧基末端的多克隆抗体，并进行了亲和纯化。MCT2抗体在免疫印迹和标记的肾脏、骨骼肌和胃上鉴定出一条46 kDa的条带，与报道的该转运蛋白的细胞表达一致。光镜下免疫细胞化学显示MCT2转运蛋白广泛分布于神经胶质细胞界膜、室管膜细胞和神经纤维层，尤其是在海马腔隙分子层和小脑分子层。标记星形胶质细胞常见于白质。这种转运体的分布在几个方面与以前报道的MCT1不同。MCT2在星形胶质细胞足突中大量分布，在脑血管系统的其他细胞，包括血管平滑肌细胞、周细胞和内皮细胞中检测不到MCT2。此外，小脑颗粒层中的MCT1标记很少，而颗粒周围的神经纤维和浦肯野细胞中的细胞突起也有MCT2标记。这一结果支持了星形胶质细胞通过运输乳酸和其他单羧酸盐在大脑能量代谢中发挥重要作用的概念。Glia 22：272-281,1998。©1998 Wiley-Liss，Inc.
The nucleotide sequence of the rat monocarboxylate transporter MCT2 was determined from brain‐derived cDNA. A polyclonal antibody was raised in chickens against the carboxyl terminal end of the deduced amino acid sequence and affinity purified. The MCT2 antibody identified a 46‐kDa band on immunoblots and labeled kidney, skeletal muscle, and stomach consistent with the reported cellular expression for this transporter. Light microscopic immunocytochemistry indicated that the MCT2 transporter was abundant in glial limiting membranes, ependymocytes, and neuropil, particularly in the lacunosum molecular layer of hippocampus and the molecular layer of cerebellum. Labeled astrocytes were commonly observed in white matter. The distribution of this transporter differed in several respects from that previously reported for MCT1. MCT2 was abundantly distributed in astrocyte foot processes and was usually not detected in other cells of the cerebrovasculature, including vascular smooth muscle cells, pericytes, and endothelium. In addition, the granular layer of cerebellum, which showed little MCT1 labeling, exhibited MCT2 labeling of cellular processes in the neuropil surrounding the granule and Purkinje cells. The results lend support to the concept that astrocytes play a significant role in cerebral energy metabolism by transporting lactate and other monocarboxylates. GLIA 22:272–281, 1998. © 1998 Wiley‐Liss, Inc.