Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS-LRR gene ZmNL in maize (Zea mays)
Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS-LRR gene ZmNL in maize (Zea mays)
复制标题
玉米 (Zea mays) 中 NBS-LRR 基因 ZmNL 的互补 DNA (cDNA) 克隆和抗丝黑病 (Sphacelotheca reiliana) 的功能验证
DOI：
10.1007/s10681-017-2083-1
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发表时间：
2017-12-01
期刊：
影响因子：
1.9
通讯作者：
Wang, Zhenhua
中科院分区：
文献类型：
--
作者：
Di, Hong;Yu, Tao;Wang, Zhenhua
核苷酸结合位点（NBS）-富含亮氨酸重复序列（LRR）基因家族是已知抗病基因中数量最多的一个，也是植物中最大的基因家族之一。本研究利用cDNA末端快速扩增法（Rapid Amplification of cDNA Ends）克隆了ZmNL的全长cDNA（GenBank登录号：KF 765443）。ZmNL的核苷酸序列包含一个3156 bp的开放阅读框，编码1051个氨基酸残基的ZmNL蛋白。该蛋白与已知的抗病蛋白同源性较高（与普通小麦LR 10同源性为84%），属于CC-NBS-LRR型R基因家族。通过农杆菌介导法将玉米泛素启动子控制的ZmNL基因导入高感丝黑穗病玉米自交系黄早4号。3个T2转基因株系的丝黑穗病发病率比野生型显著降低（降低18.38-29.40%），表明ZmNL基因在玉米中的过表达增强了这些株系对丝黑穗病菌克林特的抗性。
The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family comprises the largest number of known disease resistance (R) genes and is one of the largest gene families in plants. In the present study, the full-length cDNA ofZmNL(GenBank Accession Number KF765443) was isolated using Rapid Amplification of cDNA Ends. The nucleotide sequence ofZmNLcontains an open reading frame of 3156 bp that encodes theZmNLprotein, which is comprised of 1051 amino acid residues. This putative protein has high homology to other known resistance proteins (84% toTriticum aestivum LR10) and belongs to theCC–NBS–LRRtypeRgene family. TheZmNLgene was introduced into the maize inbred line of Huangzao4 which was highly susceptible to head smut under the control of the maize ubiquitin promoter byAgrobacterium-mediated transformation. The head smut disease incidence of 3 T2transgenic lines was significantly reduced (by 18.38–29.40%) compared with the wild type, which indicated that the overexpression ofZmNLgene in maize enhanced the resistance to the fungusSporisorium reilianum(Kühn) Clint of these plants.