Surface thiols of human lymphocytes and their changes after in vitro and in vivo activation
Surface thiols of human lymphocytes and their changes after in vitro and in vivo activation
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DOI：
10.1002/jlb.60.5.611
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发表时间：
1996-11-01
影响因子：
5.5
通讯作者：
Weber, P
中科院分区：
文献类型：
--
作者：
Lawrence, DA;Song, RJ;Weber, P
长期以来，细胞硫醇在细胞激活和增殖中起作用；然而，表面硫醇在淋巴亚群上的差异表达尚未被描述，也不知道表面硫醇在暴露于有丝分裂原或感染物后是否发生改变。在此，使用一种非必要的硫醇特异的荧光探针用于表面硫醇的流式细胞术分析，表面硫醇在静止淋巴细胞上的定量显示某些亚群表达不同浓度的表面硫醇(CD19(+)>；CD8(+)>；CD4(+))。此外，在有丝分裂激活后8小时，表面硫醇在所有亚群上均增加，这种增加可被放线菌素D或莫能菌素阻断，但不能被放线菌素D或丁硫氨酸亚磺胺抑制谷胱甘肽合成。此外，蛋白质二硫键异构酶抑制剂杆菌肽抑制表面硫醇和DNA合成的增加，HIV感染者的淋巴细胞在CD19(+)和CD4(+)细胞上显示表面硫醇增加，但CD8(+)细胞不增加，尽管细胞硫醇通常被认为在抵御氧化剂、与细胞生长相关的信号传递和细胞凋亡方面发挥作用。现在有证据表明，表面硫醇的变化似乎参与了其中一些关键的细胞反应，因此，表面硫醇的数量和可能的质量差异与淋巴系亚群之间的膜差异以及它们对氧化应激的不同敏感性相关，这表明表面硫醇在激活后保持和修饰的机制是需要进一步评估的重要细胞功能。
Cellular thiols have long been known to play a role in cell activation and proliferation; however, the differential expression of surface thiols on the lymphoid subsets had not been described, Neither was it known whether alteration of surface thiols occurs after exposure to mitogens or infectious agents, Herein, an impermeant thiol-specific fluorescent probe was employed for flow cytometric analysis of surface thiols, Quantification of surface thiols on resting lymphocytes revealed that some subsets expressed different concentrations of surface thiols (CD19(+) > CD8(+) > CD4(+)). Furthermore, surface thiols increased on all subsets by 8 h after mitogenic activation, This increase was blocked by cycloheximide or monensin but not by actinomycin D or inhibition of glutathione synthesis by buthionine sulfoximine, In addition, bacitracin, an inhibitor of protein disulfide isomerase, inhibited the increase in surface thiols and DNA synthesis, Lymphocytes from HIV-infected individuals displayed increased surface thiols on CD19(+) and CD4(+) cells but not CD8(+) cells, Although cellular thiols in general have been believed to play a role in protection against oxidants, signaling associated with cell growth, and apoptosis, there is now evidence that changes in exofacial thiols appear to be involved in some of these critical cell reactivities, Thus, quantitative and possibly qualitative differences in surface thiols correlate with membrane differences between lymphoid subsets and with their differential sensitivities to oxidative stress, which suggests that the mechanisms by which surface thiols are maintained and modified after activation are important cellular functions that need to be further evaluated.