LOW-DENSITY LIPOPROTEIN UNDERGOES OXIDATIVE MODIFICATION INVIVO
LOW-DENSITY LIPOPROTEIN UNDERGOES OXIDATIVE MODIFICATION INVIVO
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DOI：
10.1073/pnas.86.4.1372
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
WITZTUM, JL
中科院分区：
文献类型：
--
作者：
PALINSKI, W;ROSENFELD, ME;WITZTUM, JL
低密度脂蛋白(LDL)被认为必须经过氧化修饰才能产生泡沫细胞，泡沫细胞是动脉粥样硬化脂肪条纹病变的关键成分。低密度脂蛋白的氧化可能会产生氧化脂肪酸片段和载脂蛋白B之间的广泛的偶联物。我们现在提出了三条相互支持的证据来证明低密度脂蛋白在体内的氧化：(I)抗氧化低密度脂蛋白、丙二醛-赖氨酸或4-羟基十一醛-赖氨酸的抗体在低密度脂蛋白受体缺乏的兔的动脉粥样硬化病变中识别物质；(Ii)从这些兔的病变部位轻轻提取的低密度脂蛋白可被抗丙二醛结合的低密度脂蛋白的抗血清识别；(Iii)在兔和人血清中可以证明抗丙二醛-低密度脂蛋白的自身抗体(滴度从512到4096)。
It has been proposed that low density lipoprotein (LDL) must undergo oxidative modification before it can give rise to foam cell, the key component of the fatty streak lesion of atherosclerosis. Oxidation of LDL probably generates a broad spectrum of conjugates between fragments of oxidized fatty acids and apolipoprotein B. We now present three mutually supportive lines of evidence for oxidation of LDL in vivo: (i) Antibodies against oxidized LDL, malondialdehyde-lysine, or 4-hydroxynonemal-lysine recognize materials in the atherosclerotic lesions of LDL receptor-deficient rabbits; (ii) LDL gently extracted from lesions of these rabbits is recognized by an antiserum against malondialdehyde-conjugated LDL; (iii) autoantibodies against malondialdehyde-LDL (titers from 512 to > 4096) can be demonstrated in rabbit and human sera.