Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica.
Ultrastructural localization of egg-laying prohormone-related peptides in the atrial gland of Aplysia californica.
复制标题
海兔心房腺中产卵激素原相关肽的超微结构定位。
DOI：
10.1007/bf00300687
复制
发表时间：
1995
影响因子：
3.6
通讯作者：
Kurosky,A
中科院分区：
文献类型：
--
作者：
vanHeumen,WR;Nagle,GT;Kurosky,A
心房腺是一个外分泌器官，分泌到输卵管中，表达三个属于产卵激素基因家族的同源基因。虽然在神经内分泌袋细胞中的产卵激素前体的翻译后加工已被详细研究，相对较少的是已知的心房腺中的产卵激素相关基因产物的翻译后加工。结合形态学技术，包括光镜组织学和免疫细胞化学，透射电子显微镜，免疫电子显微镜被用来本地化心房腺中的产卵相关肽，并评估其分泌细胞的特征形态。这些研究结果表明，心房腺中至少有三种主要的分泌细胞（1-3型）。值得注意的是，这三种细胞类型中，只有1型是免疫反应性的抗血清对产蛋胚胎相关的前体肽。的免疫反应性研究建立了所有三个产蛋胚胎相关的前体基因表达的1型细胞，并表明，这些前体的处理也发生在这种细胞类型的分泌颗粒。还获得的证据表明，蛋白水解过程中的蛋产卵相关的前体细胞中观察到的显着不同。与囊细胞相反，心房腺的产卵囊泡相关前体的NH 2-末端和COOH-末端产物未被分类为不同类型的囊泡。
The atrial gland is an exocrine organ that secretes into the oviduct ofAplysia californicaand expresses three homologous genes belonging to the egglaying hormone gene family. Although post-translational processing of the egg-laying hormone precursor in the neuroendocrine bag cells has been examined in detail, relatively little is known about the post-translational processing of egg-laying hormone-related gene products in the atrial gland. A combination of morphologic techniques that included light-microscopic histology and immunocytochemistry, transmission electron microscopy, and immuno-electron microscopy were used to localize egg-laying hormone-related peptides in the atrial gland and to evaluate the characteristic morphology of their secretory cells. Results of these studies showed that there were at least three major types of secretory cells in the atrial gland (types 1–3). Significantly, of these three cell types, only type 1 was immunoreactive to antisera against egg-laying hormone-related precursor peptides. The immunoreactivity studies established that all three egg-laying hormone-related precursor genes are expressed in type-1 cells and indicated that the processing of these precursors also occurs within the secretory granules of this cell type. Evidence was also obtained that proteolytic processing of the egg-laying hormone-related precursors differed significantly from that observed in the bag cells. In contrast to the bag cells, the NH2-terminal and COOH-terminal products of the egg-laying hormone-related precursors of the atrial gland were not sorted into different types of vesicles.