INFECTION AND TRANSFORMATION OF BOVINE LYMPHOID-CELLS IN-VITRO BY INFECTIVE PARTICLES OF THEILERIA-PARVA
INFECTION AND TRANSFORMATION OF BOVINE LYMPHOID-CELLS IN-VITRO BY INFECTIVE PARTICLES OF THEILERIA-PARVA
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DOI：
10.1038/245101a0
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发表时间：
1973-01-01
期刊：
影响因子：
64.8
通讯作者：
PAYNE, RC
中科院分区：
文献类型：
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作者：
BROWN, CGD;STAGG, DA;PAYNE, RC
该实验室最近开发的技术可用于用从蜱虫媒介——尾鼻头蜱收集到的细小伊氏绦虫的感染性颗粒重复感染牛东海岸热。感染颗粒可以通过诱导预先饲养的感染蜱虫唾液进入含有胎牛血清的毛细管中作为体外蜱虫饲料收集1，也可以通过在Eagle的最低必需培养基中使用厄尔盐（MEM）和牛血浆白蛋白（来自牛血浆的V部分）（BPA）研磨蜱虫获得磨碎的蜱虫上清，并收集上清2。
TECHNIQUES recently developed in this laboratory can be used to infect cattle reproducibly with East Coast fever with infective particles ofTheileria parvacollected from the tick vector,Rhipicephalus appendiculatus. Infective particles can be collected either asin vitrotick feed material by inducing prefed, infected ticks to salivate into capillary tubes containing foetal calf serum1, or as ground tick supernatant obtained by grinding the ticks in Eagle's minimum essential medium with Earle's salts (MEM) supplemented with bovine plasma albumin (Fraction V from bovine plasma) (BPA) and collecting the supernatant2.