Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
Antibacterial and anti-biofilm activities of cinnamaldehyde against S. epidermidis
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DOI：
10.1016/j.micpath.2018.11.009
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发表时间：
2019-01-01
影响因子：
3.8
通讯作者：
Fernandes Junior, Ary
中科院分区：
文献类型：
--
作者：
Albano, Mariana;Crulhas, Bruno Pereira;Fernandes Junior, Ary
由于细菌对目前使用的抗生素的耐药性增加，寻找新的抗微生物药物是必要的，天然产物在这一领域发挥着重要作用。本研究的目的是评价肉桂醛对S.表皮菌株，生物膜建立预防，以及其对预先建立的生物膜的影响。最低抑菌浓度（MIC）为300 ~ 500 μ g/mL，最低杀菌浓度（MBC）为400 ~ 600 μ g/mL。生物膜抑制浓度和生物膜根除浓度值分别是抑制生长所需浓度的4倍（临床分离株）和8倍（ATCC菌株）。肉桂醛的亚抑制浓度减弱了S. epidermidis菌株在聚苯乙烯微量滴定板上进行测定。肉桂醛和利奈唑胺联合使用能够抑制S。表皮具有杀菌作用。对肉桂醛作用机制的进一步研究揭示了其对细胞膜通透性的影响，共聚焦激光扫描显微镜（CLSM）图像说明了肉桂醛对现有生物膜的分离和杀伤的影响。因此，我们的数据证实了肉桂醛能够减少S.表皮，抑制生物膜形成和根除预先形成的生物膜。
The search for new antimicrobial drugs has been necessary due to the increased bacterial resistance to antibiotics currently in use, and natural products play an important role in this field. The aim of this study was to evaluate the in vitro effect of cinnamaldehyde on S. epidermidis strains, biofilm set-up prevention, as well as its effect on pre-established biofilms. The minimum inhibitory concentration (MIC) ranged from 300 to 500 mu g/mL, and the minimum bactericidal concentration (MBC) from 400 to 600 mu g/mL. The biofilm inhibitory concentration and biofilm eradication concentration values were four-fold (clinical isolate) and eight-fold (ATCC strain) greater than the concentration required to inhibit planktonic growth. Sub-inhibitory concentrations of cinnamaldehyde attenuated biofilm formation of S. epidermidis strains on polystyrene microtiter plates. The combination of cinnamaldehyde and linezolid was able to inhibit S. epidermidis with a bactericidal effect. Further investigation of the mechanism of action of cinnamaldehyde revealed its effect on the cell membrane permeability, and confocal laser scanning microscopy (CLSM) images illustrated the impact of cinnamaldehyde in the detachment and killing of existing biofilms. Thereby, our data confirmed the ability of cinnamaldehyde to reduce bacterial planktonic growth of S. epidermidis, inhibiting biofilm formation and eradicating pre-formed biofilm.