DIVERSITY OF AGGLUTINATING PHENOTYPE, CYTOADHERENCE, AND ROSETTE-FORMING CHARACTERISTICS OF PLASMODIUM-FALCIPARUM ISOLATES FROM PAPUA-NEW-GUINEAN CHILDREN
DIVERSITY OF AGGLUTINATING PHENOTYPE, CYTOADHERENCE, AND ROSETTE-FORMING CHARACTERISTICS OF PLASMODIUM-FALCIPARUM ISOLATES FROM PAPUA-NEW-GUINEAN CHILDREN
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DOI：
10.4269/ajtmh.1994.51.45
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发表时间：
1994-07-01
影响因子：
3.3
通讯作者：
BROWN, GV
中科院分区：
文献类型：
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作者：
REEDER, JC;ROGERSON, SJ;BROWN, GV
抗原变异、细胞粘附、玫瑰花结形成和疟疾发病机制之间的关系引起了人们对来自不同社区的恶性疟原虫分离株中这些特性的多样性的极大兴趣。在这项研究中，我们扩展了以前的调查，描绘了频谱的凝集表型，粘附C32黑色素瘤细胞，人脐静脉内皮细胞（HUVEC），CD 36，细胞内粘附分子-1（ICAM-1），和玫瑰花结形成能力的一组20个恶性疟原虫分离株从巴布亚新几内亚儿童。用儿童恢复期血清和一组成人免疫血清测定凝集表型。在这一最大的同类研究中，凝集试验未能鉴定出具有相同凝集表型的任何两个分离株，这证明了该群体中存在广泛的变异抗原类型。新鲜和冻存分离株的凝集谱比较表明，试验前冻存的一般可接受性，但警告说，一些分离株可能会在此过程中经历选择和表型变化。19个菌株能够结合到至少一个的四个配体的研究，并表现出显着的变化，结合的亲和力和特异性。纯化的蛋白质ICAM-1和CD 36被证明是最有用的检测配体调查现场分离株，与18个分离株结合至少一种蛋白质和14两者。没有发现任何两个配体之间的结合分离，也没有之间的结合标准化接种物和病人的寄生虫血症的水平。研究组的所有分离株均形成轮虫（参与轮虫的培养滋养体的平均比率为14.6%）。玫瑰花结形成和CD 36结合之间缺乏相关性表明，以前报道的CD 36作为玫瑰花结形成受体的作用可能并不重要，分离自巴布亚新几内亚。
The relationship between antigenic variation, cytoadherence, rosette formation, and the pathogenesis of malaria has led to great interest in the diversity of these properties in Plasmodium falciparum isolates from different communities. In this study, we extend previous investigations by delineating the spectrum of agglutinating phenotypes, adherence to C32 melanoma cells, human umbilical vein endothelial cells (HUVEC), CD36, and intracellular adhesion molecule-1 (ICAM-1), and rosette-forming ability of a group of 20 P. falciparum isolates from Papua New Guinean children. Agglutination phenotypes were determined by using both the children's convalescent serum and a panel of adult immune sera. The wide range of variant antigenic types in the community was demonstrated by the failure of the agglutination assays to identify any two isolates with the same agglutinating phenotype in this, the largest study of its kind. Comparison of agglutination profiles from fresh and cryopreserved isolates demonstrated the general acceptability of cryopreservation before testing, but cautioned that some isolates may undergo selection and phenotypic change during the process. Nineteen isolates were able to bind to at least one of the four ligands studied and showed marked variation in both avidity and specificity of binding. The purified proteins ICAM-1 and CD36 proved to be the most useful assay ligands for investigating field isolates, with 18 isolates binding to at least one protein and 14 to both. No correlation was found between the binding of isolates to any two ligands nor between the binding of a standardized inoculum and the level of the patient's presenting parasitemia. All isolates from the study group were found to form rosettes (at a mean rate of 14.6% of cultured trophozoites involved in rosettes). A lack of correlation between rosette formation and CD36 binding suggests that the previously reported role of CD36 as a rosette formation receptor may not be important for isolates from Papua New Guinea.