Oskar protein interaction with Vasa represents an essential step in polar granule assembly
Oskar protein interaction with Vasa represents an essential step in polar granule assembly
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DOI：
10.1101/gad.10.17.2179
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发表时间：
1996-09-01
影响因子：
10.5
通讯作者：
Ephrussi, A
中科院分区：
文献类型：
--
作者：
Breitwieser, W;Markussen, FH;Ephrussi, A
果蝇卵的后极细胞质包含胚胎腹部和生殖细胞命运的决定因素。卵母细胞后极的oskar RNA诱导极胞质组装。遗传学已经揭示了另外三个基因，staufen，vasa和tudor，它们也是极浆形成所必需的。Staufen蛋白是oskar RNA定位和翻译所必需的。Vasa和Tudor依赖于Oskar蛋白定位，并且需要在后极稳定地积累Oskar蛋白。我们已经探索了这些基因产物之间的相互作用在分子水平上，并发现奥斯卡直接与瓦萨和施陶芬，在酵母双杂交试验。这些相互作用也发生在体外，并受到Oskar突变的影响，在体内废除极浆形成。最后，我们表明，在极血浆中，奥斯卡蛋白，像瓦萨和都铎，是极性颗粒的组成部分，生殖系特异性RNP结构。这些结果表明，Oskar-Vasa相互作用构成了极性颗粒组装的初始步骤。此外，我们还讨论了奥斯卡-施陶芬相互作用可能的生物学作用。
The posterior pole plasm of the Drosophila egg contains the determinants of abdominal and germ-cell fates of the embryo. Pole plasm assembly is induced by oskar RNA localized to the posterior pole of the oocyte. Genetics has revealed three additional genes, staufen, vasa, and tudor, that are also essential for pole plasm formation. Staufen protein is required for both oskar RNA localization and translation. Vasa and Tudor are localized dependent on Oskar protein and are required to accumulate Oskar protein stably at the posterior pole. We have explored interactions between these gene products at the molecular level and find that Oskar interacts directly with Vasa and Staufen, in a yeast two-hybrid assay. These interactions also occur in vitro and are affected by mutations in Oskar that abolish pole plasm formation in vivo. Finally, we show that in the pole plasm, Oskar protein, like Vasa and Tudor, is a component of polar granules, the germ-line-specific RNP structures. These results suggest that the Oskar-Vasa interaction constitutes an initial step in polar granule assembly. In addition, we discuss the possible biological role of the Oskar-Staufen interaction.