Biochemical markers of the progress of differentiation in cloned teratocarcinoma cell lines.
Biochemical markers of the progress of differentiation in cloned teratocarcinoma cell lines.
复制标题
克隆畸胎癌细胞系分化进展的生化标记。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
G. Magrane
中科院分区：
文献类型：
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作者：
E. Adamson;M. Evans;G. Magrane
单个畸胎瘤细胞的后代在体外将产生几种不同的细胞类型，并且后者显示出通过生化标准的功能分化。在所有这些研究中，对小鼠畸胎瘤细胞克隆系在分化过程中形成的组织的某些生化产物特征进行了测定。癌胚蛋白，甲胎蛋白，不合成未分化的胚胎癌（EC）细胞，但在其分化为内胚层型细胞在悬浮培养过程中合成的数量增加。α-胎蛋白被证明是内胚层细胞的产物，但不是所有的内胚层细胞都合成这种蛋白。在EC细胞或聚集体在组织培养皿中生长的进一步分化过程中，出现了其他生化产物。在主要含有神经型细胞的培养物中，乙酰胆碱酯酶的比活性增加了30倍，同时出现了小鼠大脑特有的醛缩酶同工酶。在某些培养物中，少量肌型细胞形成的标志是肌酸磷酸激酶MB同工酶的出现。生化分化一般不成熟。
The progeny of single teratocarcinoma cells will give rise to several different cell types in vitro, and the latter were shown to be functionally differentiated by biochemical criteria. In all these studies, cloned lines of mouse teratocarcinoma cells were assayed during the course of differentiation for some biochemical products characteristic of the tissues formed. The carcinoembryonic protein, alpha-foetoprotein, was not synthesized by undifferentiated embryonal carcinoma (EC) cells, but was synthesized in increasing amounts during their differentiation to endoderm-type cells in suspension culture. alpha-Foetoprotein was shown to be a product of endoderm cells, but not all endoderm cells synthesized this protein. During the course of further differentiation when EC cells or aggregates were grown in tissue-culture dishes, other biochemical products appeared. In cultures containing predominantly nerve-type cells, there was a 30-fold increase in the specific activity of acetylcholinesterase, with concomitant appearance of the aldolase isoenzyme characteristic of mouse brain. In some cultures, a small amount of muscle-type cell formation was marked by the appearance of the MB isoenzyme of creatine phosphokinase. Generally, biochemical differentiation was immature.