SPOROPOLLENIN IN CELL-WALL OF CHLORELLA AND OTHER ALGAE - ULTRASTRUCTURE, CHEMISTRY, AND INCORPORATION OF C-14-ACETATE, STUDIED IN SYNCHRONOUS CULTURES
SPOROPOLLENIN IN CELL-WALL OF CHLORELLA AND OTHER ALGAE - ULTRASTRUCTURE, CHEMISTRY, AND INCORPORATION OF C-14-ACETATE, STUDIED IN SYNCHRONOUS CULTURES
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DOI：
10.1007/bf00398011
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发表时间：
1972-01-01
期刊：
影响因子：
4.3
通讯作者：
GUNNING, BES
中科院分区：
文献类型：
--
作者：
ATKINSON, AW;JOHN, PCL;GUNNING, BES
小球藻（Chlorella fuscavar.vacuolata，剑桥211/8 p）的细胞抵制了酶降解细胞壁产生裸原生质体的努力，因此，对细胞壁的发育和组成的研究被中断。1.胞质分裂在母细胞壁内产生裸的自生孢子后，在自生孢子质膜外开始形成细胞壁，出现小的三层空斑。这些扩大，而间的自生孢子颗粒物质减少的数量，他们最终融合，产生一个完整的三层鞘周围的每个autospor.2。微纤维，纤维素酶消化，层之间的三层组件和质膜沉积。同时，母细胞壁的相应微纤维组分被消化，仅留下其抗性三层组分。3.三层组分包括一种被认为是聚合类胡萝卜素的物质，即孢粉素，基于其对极端提取方法的抗性，4.用~（14）C-脉冲处理和脉冲追踪处理的方法，发现了孢粉素生物合成的两个阶段。在同步培养的细胞周期中，每隔一段时间乙酸盐。一个与含孢粉素的三层壁成分的形成相一致，另一个是早于6-8小时，而细胞处于有丝分裂。5.在所测的5株小球藻中，只有1株有孢粉素，而在栅藻中只有1株有，在无绿藻中有2株有。6.对上述藻类细胞壁结构的研究表明，7.对文献的调查支持了这一假设，并进一步表明，合成孢粉素的能力与产生次生类胡萝卜素的能力有关。
Cells ofChlorella fuscavar.vacuolata(Cambridge 211/8p) resisted efforts aimed at producing naked protoplasts by enzymatic degradation of the cell wall, and a study of the development and composition of the wall was therefore undertaken.1.After cytokinesis has produced naked autospores within the mother cell wall, cell wall formation commences outside the autospore plasma membrane with the appearance of small trilaminar plaques. These enlarge while inter-autospore granular material diminishes in quantity, and they eventually fuse to produce a complete trilaminar sheath around each autospore.2.A microfibrillar, cellulase digestible, layer is deposited between the trilaminar component and the plasma membrane. Meanwhile the corresponding microfibrillar component of the mother cell wall is digested leaving only its resistant trilaminar component.3.The trilaminar component includes a substance considered to be the polymerized carotenoid, sporopollenin, on the basis of its resistance to extreme extraction procedures including acetolysis, and its infra red absorption spectrum.4.Two phases of sporopollenin biosynthesis were detected by means of pulse and pulse-chase treatments with14C-acetate at intervals during the cell cycle in synchronous cultures. One coincides with the formation of the sporopollenin-containing trilaminar wall component, and the other is 6–8 hours earlier while the cells are in karyokinesis. The former yields labelled sporopollenin directly and the latter probably represents formation of a precursor.5.Of five other strains ofChlorellatested, only one possesses sporopollenin, and so does oneScenedesmusand two out of three strains ofPrototheca.6.Examination of the wall structure of the above algae suggest a relationship between the presence of sporopollenin and the development of an outer, trilaminar wall component.7.A survey of the literature gives support to this hypothesis and further suggests that the ability to synthesise sporopollenin is related to the ability to produce secondary carotenoids.8.The significance of the findings is discussed.