Toward fluorescence nanoscopy
Toward fluorescence nanoscopy
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DOI：
10.1038/nbt895
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发表时间：
2003-11-01
影响因子：
46.9
通讯作者：
Hell, SW
中科院分区：
文献类型：
--
作者：
Hell, SW
一个多世纪以来，聚焦光学显微镜的分辨率一直被限制在焦面上的衍射到180 nm，沿光轴的衍射到500 nm。最近，有报道称，显微镜可以将活细胞的轴向分辨率提高三到七倍。此外，出现了一系列概念，完全克服了衍射障碍。它的第一个指数，受激发射耗尽显微镜，到目前为止已经显示出分辨率到28纳米。依靠饱和的光学跃迁，这些概念仅受可达到的饱和水平的限制。由于强饱和在低光强下应该是可行的，因此聚焦光的纳米尺度成像可能比以往任何时候都更接近。
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, microscopes have been reported that provide three- to sevenfold improved axial resolution in live cells. Moreover, a family of concepts has emerged that overcomes the diffraction barrier altogether. Its first exponent, stimulated emission depletion microscopy, has so far displayed a resolution down to 28 nm. Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level. As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than ever.