Identification of the AQP members involved in abiotic stress responses from Arabidopsis
Identification of the AQP members involved in abiotic stress responses from Arabidopsis
复制标题
拟南芥非生物胁迫反应中 AQP 成员的鉴定
DOI：
10.1016/j.gene.2017.12.048
复制
发表时间：
2018-03-10
期刊：
影响因子：
3.5
通讯作者：
Gong, Ya-Ming
中科院分区：
文献类型：
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作者：
Feng, Zhi-Juan;Xu, Sheng-Chun;Gong, Ya-Ming
水通道蛋白(Aquaporins，AQP)是一个高度多样化的水通道蛋白家族，在植物生长发育和逆境生理中发挥着重要的生物学功能。在拟南芥中，35个AQP被分为四个亚家族(PIPs、TIPS、NIPs和SIPs)。然而，对不同亚家族AQP的作用的了解仍然有限。在这里，我们基于现有的基因芯片数据，探索了所有水通道蛋白在不同组织或不同非生物胁迫下的染色体位置、基因结构和表达模式。组织表达分析表明，不同的水通道蛋白在根、叶、花和种子等组织中的表达模式不同。胁迫条件下的表达谱显示，大多数水通道蛋白对渗透胁迫、盐胁迫和干旱胁迫都有响应。表型和生理鉴定表明，与野生型相比，Tip2；2功能缺失突变体对非生物胁迫(甘露醇、氯化钠和聚乙二醇)的敏感性较低，从发芽率、根生长、成活率、离子渗漏、丙二醛(MDA)和脯氨酸含量等方面可以看出这一点。在非生物胁迫条件下，突变体TIP2；2调节了SOS1、SOS2、SOS3、DREB1A、DREB2A和P5CS1的转录水平。本研究为进一步鉴定拟南芥中与胁迫相关的候选水通道蛋白奠定了基础。
Aquaporins (AQPs) constitute a highly diverse family of water channel proteins that play crucial biological functions in plant growth and development and stress physiology. In Arabidopsis, 35 AQPs are classified into four subfamilies (PIPs, TIPs, NIPs and SIPs). However, knowledge about the roles of different subfamily AQPs remains limited. Here, we explored the chromosomal location, gene structure and expression patterns of all AQPs in different tissues or under different abiotic stresses based on available microarray data. Tissue expression analysis showed that different AQPs had various expression patterns in tissues (root, leaf, flower and seed). Expression profiles under stress conditions revealed that most AQPs were responsive to osmotic, salt and drought stresses. Phenotypic and physiological identification showed that Tip2;2 loss-of-function mutant exhibited less sensitive to abiotic stresses (mannitol, NaCl and PEG) compared with wild-type, as evident by analysis of germination rate, root growth, survival rate, ion leakage, malondialdehyde (MDA) and proline contents. Mutant of TIP2;2 modulated the transcript levels of SOS1, SOS2, SOS3, DREB1A, DREB2A and P5CS1, under abiotic stress conditions. This study provides a basis for further functional identification of stress-related candidate AQPs in Arabidopsis.