BMP signaling in the epiblast is required for proper recruitment of the prospective paraxial mesoderm and development of the somites
BMP signaling in the epiblast is required for proper recruitment of the prospective paraxial mesoderm and development of the somites
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DOI：
10.1242/dev.02552
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发表时间：
2006-10-01
期刊：
影响因子：
4.6
通讯作者：
Mishina, Yuji
中科院分区：
文献类型：
--
作者：
Miura, Shigeto;Davis, Shannon;Mishina, Yuji
BMPR1A编码骨形态发生蛋白(BMPs)的IA型BMP受体，包括2和4。在这里，我们使用小鼠胚胎(BmPR-More胚胎)上胚中BMPR1A的嵌合体失活来评估该基因在中胚层发育中的功能。与不能原肠形成的BMPR1A缺失胚胎不同，Bmpr-More胚胎启动原肠形成，但预期的轴旁中胚层细胞向原始条纹的招募被推迟。这种延迟导致原始条纹内具有旁轴中胚层特征的细胞更近距离的分布，导致体细胞中胚层的侧向扩张形成多个柱状。抑制成纤维细胞生长因子信号可以恢复预期的中轴旁中胚层招募的正常时间，并部分挽救体节的发育。这表明BMP和成纤维细胞生长因子信号在近轴向中胚层发育过程中起拮抗作用。
Bmpr1a encodes the BMP type IA receptor for bone morphogenetic proteins (BMPs), including 2 and 4. Here, we use mosaic inactivation of Bmpr1a in the epiblast of the mouse embryo (Bmpr-MORE embryos) to assess functions of this gene in mesoderm development. Unlike Bmpr1a-null embryos, which fail to gastrulate, Bmpr-MORE embryos initiate gastrulation, but the recruitment of prospective paraxial mesoderm cells to the primitive streak is delayed. This delay causes a more proximal distribution of cells with paraxial mesoderm character within the primitive streak, resulting in a lateral expansion of somitic mesoderm to form multiple columns. Inhibition of FGF signaling restores the normal timing of recruitment of prospective paraxial mesoderm and partially rescues the development of somites. This suggests that BMP and FGF signaling function antagonistically during paraxial mesoderm development.